In vitro testing of fresh and lyophilized reconstituted human and baboon platelets

Transfusion
C Robert ValeriGina Ragno

Abstract

Studies have been performed on human fresh, liquid-preserved, and cryopreserved platelets (PLTs) to assess PLT-adhesive surface receptors, PLT membrane procoagulant activity, PLT aggregation, and thromboxane production. Lyophilization has been developed as a method to preserve PLTs. This study was performed to evaluate these measurements on human and baboon fresh and lyophilized reconstituted PLTs. In both human and baboon fresh and lyophilized PLTs, aggregation response and PLT production of thromboxane A2 were measured after stimulation, and PLT surface markers P-selectin, glycoprotein (GP) Ib, GPIIb-IIIa, and factor (F) V were measured before and after stimulation. Fresh PLTs responded to the dual agonists arachidonic acid and adenosine diphosphate (ADP) to aggregate and produce thromboxane A2, and in both the PLT surface markers P-selectin and GPIIb-IIIa increased and GPIb decreased after stimulation. Neither human nor baboon lyophilized reconstituted PLTs aggregated to dual agonists, and neither produced thromboxane A2, increased PLT surface markers P-selectin or GPIIb-IIIa, or decreased PLT GPIb after stimulation. Nevertheless, after recalcification the lyophilized reconstituted PLTs accumulated FV to a significantly grea...Continue Reading

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Citations

Feb 23, 1999·British Journal of Haematology·A D MichelsonC R Valeri
Aug 24, 2011·The Journal of Trauma·Andrew P Cap, Jeremy G Perkins
Dec 6, 2008·Transfusion Medicine Reviews·Jelena L HolovatiJason P Acker
Jun 29, 2011·International Journal of Laboratory Hematology·N AhmadzadehM R Khorramizadeh
Nov 12, 2013·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·A B SanthakumarI Singh
Mar 8, 2006·Seminars in Fetal & Neonatal Medicine·Courtney Thornburg, Steven Pipe
Apr 25, 2008·Platelets·Thomas H FischerTimothy C Nichols
Sep 24, 2020·International Journal of Molecular Sciences·Isabel AndiaNicola Maffulli
Jun 3, 2021·Journal of Clinical Medicine·Annina CapraruBehrouz Mansouri Taleghani

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