In vitro toxicity of photodynamic antimicrobial chemotherapy on human keratinocytes proliferation.

Lasers in Medical Science
Mario MigliarioFilippo Renò

Abstract

This in vitro experimental study has been designed to assess the effects of photodynamic antimicrobial chemotherapy (PACT) on human keratinocytes proliferation. Human keratinocytes (HaCaT) monolayers (∼0.5 cm(2)) have been irradiated with 635 nm red laser light with a fluence of 82.5 or 112.5 J/cm(2) in the absence or presence of toluidine (TB). Cell proliferation, monolayer area coverage, cytokeratin 5 (K5) and filaggrin (Fil) expression, and metalloproteinase (MMP)-2 and MMP-9 activity were measured after 72 h from laser irradiation. HaCaT proliferation was reduced by TB staining. Cell exposure to both low- and high-fluence laser irradiation in both presence and absence of TB staining reduced their proliferation and monolayer area extension. Moreover both laser treatments were able to reduce K5 and Fil expression and MMP-9 production in keratinocytes not treated with TB. These data indicate that PACT could exert toxic effects on normal proliferating keratinocytes present around parodontal pockets. The observed reduced cell proliferation along with a reduced production of enzymes involved in wound healing could alter the clinical outcome of the patients treated with PACT.

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Citations

Mar 7, 2014·Lasers in Medical Science·Mario MigliarioFilippo Renò
Jun 18, 2016·Journal of Diabetes Research·Sandra Matabi AyukNicolette Nadene Houreld
Jun 14, 2016·Journal of Photochemistry and Photobiology. B, Biology·Sandra M AyukNicolette N Houreld
Oct 13, 2017·Journal of Biophotonics·Sandra M AyukNicolette N Houreld
Jun 27, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Lia Mara Grosso NevesMarcia Regina Cominetti
Jun 4, 2019·Lasers in Medical Science·Priscila Thaís Rodrigues de AbreuTarcília Aparecida Silva

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