Abstract
Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Trificum monococcum with contrasting storage protein compositions and bread-making characteristics were found unable to agglutinate K562(S) cells even at a peptide concentration as high as 14 g/L, agglutination being strongly correlated with toxicity in celiac disease. When fractionated by affinity chromatography on Sepharose-6B coupled with mannan, peptic-tryptic digests separated into three fractions. Fraction C peptides were shown to agglutinate K562(S) cells, whereas peptides in fractions A and B and in the mixed fraction B + C were inactive, suggesting that fraction B contains "protective" peptides that interfere with toxic peptides in fraction C in their agglutinating activity. These results offer an opportunity to study the biochemical and genetic bases of wheat toxicity at the diploid level. Moreover, the reduced toxicity, if any, of Triticum monococcum in the celiac disease, along with the good grain characteristics of some "monococcum" accessions, greatly increases the economical prospects of this wheat species.
Citations
Sep 25, 1999·Diabetes/metabolism Research and Reviews·A K Kumagai
Dec 24, 2008·Molecular Genetics and Genomics : MGG·Patrizia VaccinoBenjamin Kilian
Nov 1, 2002·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·N E Pogna
Dec 5, 2013·The Journal of Laryngology and Otology·Z ShenJ Guo
Oct 20, 2000·Pancreas·X Z DingT E Adrian
Oct 5, 2007·Journal of Gastroenterology and Hepatology·Olimpia VincentiniNorberto Edgardo Pogna
May 28, 2013·BMC Gastroenterology·Barbara ZaniniAlberto Lanzini
Feb 1, 2014·Nutrients·Carmela LamacchiaCarmen Gianfrani
Sep 22, 2009·Gastroenterology·Detlef SchuppanDonatella Barisani
Sep 12, 2013·Journal of the Science of Food and Agriculture·Alyssa Hidalgo, Andrea Brandolini
May 29, 2015·Molecular Nutrition & Food Research·Carmen GianfraniGianfranco Mamone
Apr 5, 2015·European Journal of Nutrition·Barbara ZaniniAlberto Lanzini
Mar 8, 2013·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Tanja ŠuligojPaul J Ciclitira
Jul 5, 2016·Food Chemistry·Giuseppe IacominoGianfranco Mamone
Apr 28, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Olimpia VincentiniMassimo De Vincenzi
Mar 27, 2018·Molecular Nutrition & Food Research·Vera Rotondi AufieroGiuseppe Mazzarella
Mar 3, 2018·International Journal of Food Sciences and Nutrition·Gianfranco Mamone, Giuseppe Iacomino
Oct 25, 2006·Scandinavian Journal of Gastroenterology·Daniela PizzutiDiego Martines
Mar 3, 2020·Frontiers in Nutrition·Natasha SharmaMonika Garg
Nov 9, 2012·The American Journal of Clinical Nutrition·Carmen GianfraniGiuseppe Mazzarella
Oct 18, 2020·Healthcare·Diana Di LibertoMarianna Lauricella
May 1, 2021·Foods·Francesca ColomboPatrizia Restani