In vitro trans translation mediated by alanine-charged 10Sa RNA

Journal of Molecular Biology
H HimenoA Muto

Abstract

10Sa RNA is a bacterial small stable RNA, in which the 5' and 3'-terminal sequences can be folded into a tRNA-like secondary structure which can be aminoacylated with alanine. It was found that Escherichia coli 10Sa RNA facilitated the incorporation of alanine, tyrosine, aspartic acid and glutamic acid, but not valine, isoleucine, serine or arginine, into the growing polypeptide in vitro, depending on poly (U)-directed poly-phenylalanine synthesis. This result indicates that 10Sa RNA functions as an mRNA for the tag-peptide which has been found to be attached to the C termini of truncated polypeptides synthesized in vivo. Aminoacylation with alanine was required for tag-specific amino acid incorporation and for efficient association of 10Sa RNA with the ribosome, indicating that 10Sa RNA also functions as an alanine tRNA in the tag-peptide synthesis. The dual function of 10Sa RNA both as an mRNA and as a tRNA in vitro strongly supports the trans translation hypothesis.

References

Jul 2, 1979·Molecular & General Genetics : MGG·B K Ray, D Apirion
Oct 16, 1992·Cell·D A Shub, H Goodrich-Blair
Jan 11, 1992·Nucleic Acids Research·J S Tyagi, A K Kinger
Sep 1, 1991·Journal of Bacteriology·Y Komine, H Inokuchi
Jul 1, 1991·Journal of Molecular Recognition : JMR·K TamuraM Shimizu
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T ObaS Osawa
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y KomineH Inokuchi
Feb 16, 1996·Science·S Jentsch
Feb 29, 1996·Nature·J F Atkins, R F Gesteland
Jun 1, 1996·Nature Structural Biology·B FeldenR F Gesteland
Mar 1, 1996·Journal of Biochemistry·Y KomineH Inokuchi
Dec 16, 1996·FEBS Letters·T TadakiA Muto
Jan 1, 1996·Biochimie·B FeldenR F Gesteland
Mar 1, 1996·Science·J Brosius

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Citations

Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A MutoH Himeno
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ai FujiharaAkira Muto
Jan 25, 2003·European Journal of Biochemistry·Victor G Stepanov, Jens Nyborg
Apr 29, 1999·Nucleic Acids Research·C ZwiebJ Wower
Mar 28, 2002·Nucleic Acids Research·Kyoko Hanawa-SuetsuguAkira Muto
Aug 6, 2004·Nucleic Acids Research·Takayuki KonnoHyouta Himeno
Aug 25, 2004·Nucleic Acids Research·Stephen M Sharkady, Kelly P Williams
Oct 6, 2005·Nucleic Acids Research·Krisana AsanoHyouta Himeno
Oct 26, 2007·Nucleic Acids Research·Daisuke KuritaHyouta Himeno
Mar 21, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A DanchinP Nitschké
Jun 8, 2001·Journal of Bacteriology·T Wiegert, W Schumann
Jun 17, 2005·BMC Molecular Biology·Jody BurksJacek Wower
Jan 22, 2011·Journal of Nucleic Acids·Daisuke KuritaHyouta Himeno
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher S HayesRobert T Sauer
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Lin-ChaoY T Lin
Aug 31, 2000·Annual Review of Biochemistry·A J HerrR F Gesteland
May 6, 2003·Annual Review of Microbiology·Jeffrey H Withey, David I Friedman
Apr 30, 2014·Frontiers in Genetics·Hyouta HimenoAkira Muto
Apr 10, 2007·Biochemical and Biophysical Research Communications·Michael O'Connor
Mar 21, 2002·FEBS Letters·Yoshihiro Shimizu, Takuya Ueda
Jan 1, 2004·Toxicology Mechanisms and Methods·Supratim Choudhuri
Dec 15, 2004·The Journal of Biological Chemistry·Yannick JacobKelly P Williams
Aug 5, 2008·Molecular Microbiology·Teppei AbeAkira Muto
Oct 17, 1998·DNA Sequence : the Journal of DNA Sequencing and Mapping·A Op De BekkeJ Brosius
Apr 18, 2002·FEBS Letters·Ken-ichi ItoHyouta Himeno
May 20, 2003·The Journal of Biological Chemistry·Toshiharu TakahashiHyouta Himeno

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