PMID: 9525621Apr 3, 1998Paper

In vitro transcription of pe38/polyhedrin hybrid promoters reveals sequences essential for recognition by the baculovirus-induced RNA polymerase and for the strength of very late viral promoters

Journal of Virology
Ruud Mans, D Knebel-Mörsdorf

Abstract

In vitro transcription was used to analyze the promoter specificity of the alpha-amanitin-resistant RNA polymerase that is induced late during infection of Autographa californica multicapsid nuclear polyhedrosis virus. By modifying the preparation of crude nuclear extracts, we have established an assay that permits differentiation between weak late and strong very late viral promoters. The virus-induced RNA polymerase initiates at a TAAG sequence motif in both late and very late promoters. Based on the sensitivity of our in vitro transcription system, we have investigated the sequences responsible for a functional TAAG motif and their putative role with respect to the strength of very late promoters. By constructing hybrid promoters between the early pe38 and the very late polyhedrin promoters, we demonstrated that the replacement of 7 nucleotides upstream of the nonfunctional TAAG sequences in the pe38 promoter with the corresponding sequences of the polyhedrin promoter was sufficient for recognition by the virus-induced RNA polymerase. The strength of the very late polyhedrin promoter was established after replacing the 5' untranslated sequences of the pe38 promoter by those of the polyhedrin promoter in addition to the 7 nuc...Continue Reading

References

Jun 1, 1992·Journal of Virology·L A Guarino, M Smith
Jul 5, 1992·Journal of Molecular Biology·G A KassavetisE P Geiduschek
Feb 1, 1991·Journal of Virology·R Krappa, D Knebel-Mörsdorf
May 1, 1991·Virology·D D CarsonL A Guarino
Jan 1, 1990·Annual Review of Entomology·G W Blissard, G F Rohrmann
Jan 1, 1989·The Journal of General Virology·U Weyer, R D Possee
Jul 12, 1988·Biochemistry·H MargalitR L Jernigan
Aug 1, 1986·The Journal of General Virology·G F Rohrmann
Mar 25, 1994·Gene·T D Morris, L K Miller
Oct 1, 1993·Journal of Virology·D Becker, D Knebel-Mörsdorf
Apr 1, 1993·Molecular Microbiology·J A Jaehning
Mar 19, 1996·Biochemistry·J SantaLuciaP A Seneviratne
Jun 15, 1996·Nucleic Acids Research·M Marilley, P Pasero

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Citations

Jul 1, 2006·Journal of Virology·Simon P DuffyDavid B Levin
Jan 15, 2005·Journal of Virology·Toni-Ann Mistretta, Linda A Guarino
Sep 19, 2014·PLoS Genetics·Gaelen R BurkeMichael R Strand
Jun 29, 1999·Biological Chemistry·R M Mans, D Knebel-Mörsdorf
Jun 18, 2003·The Journal of General Virology·Jane S TitteringtonA Lorena Passarelli
Jul 10, 1999·Journal of Virology·E A Prikhod'ko, L K Miller
Sep 12, 1998·Journal of Virology·L A GuarinoW Dong
Jun 5, 2021·Protein Expression and Purification·Carissa GroseDominic Esposito

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