PMID: 6306569Jun 25, 1983Paper

In vitro transcription of the cloned chromosomal crystal gene from Bacillus thuringiensis

Nucleic Acids Research
A F KlierG Rapoport


We have determined the conditions required for in vitro transcription of the cloned chromosomal crystal gene from Bacillus thuringiensis using either the homologous vegetative RNA polymerase or a sporulation specific form of this enzyme. The gene is actively transcribed by the latter enzyme (form II) but not by the vegetative one. Evidence for a specific recognition between the form II enzyme and the promotor site of the crystal gene was obtained by binding experiments. They showed that the binding is increased by the presence of some additional factors, which change the specificity of the vegetative core-enzyme. The sequence of the promoter has been determined and the start-point of the transcription deduced. Two hexanucleotide sequences, TACAAT and CCTACG, centered at - 10 and - 35 bp are present, but are somewhat different from the consensus sequences previously described in other bacilli.


Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B B JonesW S Reznikoff
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C Talkington, J Pero
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Feb 25, 1982·Nucleic Acids Research·P Cossart, B Gicquel-Sanzey
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G A HeldS A Minnich
Sep 1, 1981·Cell·R Losick, J Pero
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H E Schnepf, H R Whiteley

Related Concepts

CTGCAG-specific type II deoxyribonucleases
Polymorphism, Crystallization
DNA Restriction Enzymes
Transcription, Genetic
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