PMID: 2116009Aug 1, 1990Paper

In vitro transcription of two rRNA genes of the archaebacterium Sulfolobus sp. B12 indicates a factor requirement for specific initiation

Proceedings of the National Academy of Sciences of the United States of America
U HüdepohlW Zillig

Abstract

We describe a cell-free transcription system for the archaebacterium Sulfolobus sp. B12 that specifically initiates transcription at the 5S rRNA-encoding DNA and the 16S/23S rRNA-encoding DNA promoters of the same species. With this crude extract system, specific initiation was absolutely dependent on the box A motif, a highly conserved promoter element in archaebacteria located approximately 25 base pairs upstream of transcription initiation sites. In vitro transcription of the rRNA genes by purified RNA polymerase, however, resulted in semi-specific, box A-independent initiation, indicating that factor(s) in the crude extract were necessary for the highly specific box A-dependent transcription. Fractionation of the cell-free extract by sucrose-gradient centrifugation resulted in the identification of a low molecular weight fraction complementing purified RNA polymerase to an extract-like specificity.

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Citations

Jan 1, 1995·World Journal of Microbiology & Biotechnology·M CiaramellaM Rossi
Jul 1, 1995·Trends in Genetics : TIG·P BaumannS P Jackson
Jul 1, 1995·Bio/technology·M W AdamsR M Kelly
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W D ReiterW Zillig
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·P J Keeling, W F Doolittle
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·D LangerW Zillig
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S A QureshiS P Jackson
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·J WettachM Thomm
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Dec 18, 2004·Nucleic Acids Research·Alessandro A SartoriJosef Jiricny
Sep 16, 2006·Nucleic Acids Research·Alexandra KesslerDavid Prangishvili
Aug 1, 2012·Nucleic Acids Research·Magdalena N WojtasNicola G A Abrescia
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S D Bell, S P Jackson
Apr 7, 2010·Journal of Bacteriology·Junaid Iqbal, Sohail A Qureshi
Aug 7, 2007·Journal of Bacteriology·Gabriella FiorentinoSimonetta Bartolucci
Jul 22, 1991·FEBS Letters·U HüdepohlW Zillig
Sep 17, 2013·Nature Reviews. Microbiology·Sonja-Verena AlbersChrista Schleper
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May 21, 2011·FEMS Microbiology Letters·Melissa DeYoungKenneth M Stedman
Apr 1, 1995·Journal of Bacteriology·J R Palmer, C J Daniels

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