In vitro transformation of mouse testis cells by oncogene transfection

Biology of Reproduction
Hiroko MorimotoTakashi Shinohara

Abstract

Germ cell tumors (GCTs) are unique in that they exhibit diverse biological characteristics and pathological features. Although several in vivo GCT models are available, studies on GCTs are hampered because in vivo development of GCTs is time consuming and prevents a detailed molecular analysis of the transformation process. Here we developed a novel strategy to transform mouse testis cells in vitro. Lentivirus-mediated transfection of dominant negative Trp53, Myc, and activated Hras1 into a CD9-expressing testis cells caused tumorigenic conversion in vitro. Although these cells resembled embryonic stem (ES) cells, they were aneuploid and lacked Nanog expression, which is involved in the maintenance of the undifferentiated state in ES cells. Euploid ES-like cells were produced by transfecting the Yamanaka factors (Pou5f1, Myc, Klf4, and Sox2) into the same cell population. Although these cells expressed Nanog, they were distinct from ES cells in that they expressed CD44, a cancer stem cell antigen. Both treatments induced similar changes in the DNA methylation patterns in differentially methylated regions of imprinted genes. Moreover, despite the differences in their phenotype and karyotype, both cell types similarly produced mi...Continue Reading

References

Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L H LooijengaJ W Oosterhuis
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hannu Sariola, Xiaojuan Meng
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Dec 12, 2003·Cancer Cell·Sharon GidekelEli Pikarsky
Feb 6, 2004·The Journal of Biological Chemistry·Naoko HattoriKunio Shiota
Dec 29, 2004·Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Mar 2, 2005·Nature Reviews. Cancer·J Wolter Oosterhuis, Leendert H J Looijenga
Sep 8, 2005·Seminars in Cell & Developmental Biology·Håkan AxelsonSven Påhlman
Dec 27, 2006·Cancer Cell·Beth A A WeaverDon W Cleveland
Feb 24, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Engin UlukayaMehmet Karadag
Feb 28, 2007·Current Opinion in Genetics & Development·Neil J GanemDavid Pellman
Dec 21, 2007·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jan 17, 2008·Nature Reviews. Genetics·Hiroyuki Sasaki, Yasuhisa Matsui
Jan 18, 2008·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Dec 17, 2008·Current Molecular Medicine·Rachid MarhabaMargot Zoeller
Oct 31, 2009·The International Journal of Developmental Biology·Si B SonneEwa Rajpert-De-Meyts
Nov 26, 2009·PloS One·Hiroko MorimotoTakashi Shinohara
Mar 18, 2011·Nature Reviews. Cancer·Duncan GilbertJanet Shipley

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Citations

Oct 9, 2013·Annual Review of Cell and Developmental Biology·Mito Kanatsu-Shinohara, Takashi Shinohara
Sep 14, 2013·Genes & Development·Seiji TakashimaTakashi Shinohara
Aug 1, 2015·The Journal of Reproduction and Development·Takashi TanakaTakashi Shinohara
Apr 11, 2018·Stem Cells and Development·Yanmin FengChunsheng Han
Dec 23, 2016·Genes & Development·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 30, 2019·International Journal of Molecular Sciences·Leendert H J LooijengaMuhammad T Idrees

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Methods Mentioned

BETA
transgenic
transfecting
transfection
PCR

Software Mentioned

ImageGauge
COBRA
Photoshop

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