PMID: 6162449Aug 1, 1980Paper

In vitro translation of maize ADH: evidence for the anaerobic induction of mRNA

Biochemical Genetics
R J FerlD Schwartz

Abstract

Total cellular RNA from anaerobically stressed maize seedling roots was used to stimulate in vitro translation of authentic maize alcohol dehydrogenase (ADH) in a rabbit reticulocyte lysate system. Total products from such reactions were displayed on NEPHGE-SDS two-dimensional gels and the Adhl-specific translation products were identified by using RNA from sib seedlings segregating for Adhl charge and size variants. The application of a rapid RNA isolation procedure allowed the efficient isolation of biologically active RNAs from small amonts of seedling material. Maize ADHs translated in vitro are identical in size to in vivo ADH. Further, no ADH was detected in the products of an in vitro translation reaction stimulated by total RNA from aerobically grown seedlings. This suggests that induction of ADH protein by anaerobic stress is accomplished by production of Adh mRNA rather than activation of sequestered mRNA. The mRNAs for maize ADH1 and ADH2 are among a small class of mRNAs induced during anaerobiosis.

References

Sep 1, 1978·Developmental Biology·G L WaringA P Mahowald
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T G WarrenA P Mahowald
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y KaufmannS Penman
Jan 1, 1973·Biochemical Genetics·M Freeling, D Schwartz

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Citations

Aug 11, 1992·Plant Molecular Biology·W L McKendree, R J Ferl
Sep 1, 1986·Plant Molecular Biology·B RicardA Pradet
Jun 1, 1987·Molecular & General Genetics : MGG·E E Irish, D Schwartz
Oct 1, 1986·Biochemical Genetics·R BrzezinskiJ P Thirion
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W L GerlachW J Peacock
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L J Rowland, J N Strommer
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C Chang, E M Meyerowitz
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A L PaulR J Ferl
May 1, 1983·Plant Physiology·L AspartA Pradet
Nov 1, 1989·Plant Physiology·J JohnsonM C Drew
Jan 1, 1981·Environmental Health Perspectives·D Schwartz
Jan 1, 1986·Developmental Genetics·G L Matters, J G Scandalios
Jan 1, 1981·Environmental Health Perspectives·R A NilanA Kleinhofs

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