Apr 25, 1980

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA

Nucleic Acids Research
J P Liautard, J M Egly


We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated although free mRNP washed with 0.5 M KC1 can direct polypeptide synthesis. Furthermore, the 0.5 M KC1 wash possesses a factor which inhibits the translation of 0.5 M KC1 washed free mRNP as well as globin mRNA naked mRNA from plasmocytoma, or Hela cells. We also demonstrated that native free mRNP are able to form a complex with ribosomal subunits in the presence of initiation factors. This indicates that inhibition of translation by the 0.5 M KC1 wash occurs either at some point after initiation complex formation or at the elongation step.

Mentioned in this Paper

Complex (molecular entity)
Transcription Initiation
Protein Biosynthesis
Metabolic Inhibition
HeLa Cells
Poly(A) Tail

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