In vitro transplantation of fetal human retinal pigment epithelial cells onto human cadaver Bruch's membrane

Experimental Eye Research
Alessandro CastellarinMarco A Zarbin

Abstract

Retinal pigment epithelium transplantation has been proposed as adjunctive treatment for age-related macular degeneration following surgical excision of choroidal neovascular membranes. The goal of this study was to develop a model to evaluate retinal pigment epithelium transplantation onto human Bruch's membrane in vitro. We investigated the ability of cultured fetal human retinal pigment epithelium to colonize human cadaver Bruch's membrane, determined the incubation time needed to form a monolayer and to exhibit apical microvilli and tight junctions, and assessed the production of basement membrane. Freshly enucleated (less than 48 hours old) human eyes were cut through the pars plana, and the anterior segment, vitreous, and retina were removed. The native retinal pigment epithelium was debrided with a surgical sponge. Bruch's membrane and choroid at the macula were trephined with a 7.0 mm diameter trephine and then incubated with 1/2 ml of Dulbecco's modified Eagle's medium +15% fetal calf serum+basic fibroblast growth factor (1 ng ml-1), and fetal human retinal pigment epithelium at a concentration of 242,000 cells ml-1. Specimens were incubated for 1, 4, 6, 8, 12, or 24 hours. The specimens were fixed in half strength Kar...Continue Reading

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Citations

Nov 5, 2011·Regenerative Medicine·Sharon L MasonCarl M Sheridan
Jun 20, 2003·Current Eye Research·Lucian V Del PrioreHenry J Kaplan
Oct 9, 2007·Progress in Retinal and Eye Research·Lyndon da CruzPete Coffey
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Jan 27, 2005·Experimental Eye Research·Vamsi K GullapalliMarco A Zarbin
Apr 22, 2015·Progress in Retinal and Eye Research·Tyler W Stradleigh, Andrew T Ishida
Jan 15, 2014·Progress in Retinal and Eye Research·Steven PennockAndrius Kazlauskas
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Jun 8, 2001·Progress in Retinal and Eye Research·R D LundJ M Lawrence
Dec 12, 2003·Experimental Eye Research·Hao WangMarco A Zarbin

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