In vitro up-regulation of HLA-G using dexamethasone and hydrocortisone in first-trimester trophoblast cells of women experiencing recurrent miscarriage

Tissue Antigens
A AkhterS Agrawal

Abstract

The trophoblast cells at the maternal-fetal interface express an unusual combination of human leukocyte antigen (HLA)-C, HLA-E and HLA-G. Altered expression of HLA-G on the extravillous cytotrophoblast has been implicated in the etiology of recurrent miscarriages (RMs). We have assessed HLA-G expression in extravillous cytotrophoblast in cell cultures prepared from RM patients and compared with those of first-trimester voluntarily terminated normal pregnancies (control). Glucocorticoids, dexamethasone and hydrocortisone were examined for their role in modulation of the HLA-G expression. HLA-G promoter and 3'UTR variants were investigated for their effect on the transcription of HLA-G. Cultured cytotrophoblast cells from the first-trimester RM patients were treated with dexamethasone and hydrocortisone (dose concentration 0-1000 ng/ml). HLA-G gene transcription was determined by semiquantitative and quantitative real-time polymerase chain reaction (RT-PCR), while protein expression was determined by a specific enzyme-linked immunosorbent assay (ELISA), flow cytometry and western blot analyses. HLA-G polymorphisms were detected by PCR and/or sequence-based typing. Low level of HLA-G was observed in untreated trophoblast cells obt...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1991·Immunology Today·A King, Y W Loke
Apr 13, 1990·Science·S KovatsR DeMars
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·N Rouas-FreissE D Carosella
Apr 6, 1999·The Journal of Experimental Medicine·S Rajagopalan, E O Long
May 18, 1999·International Immunology·P MoreauP Paul
May 29, 2000·Human Immunology·K A PfeifferH Grosse-Wilde
Jul 21, 2000·Lancet·N LilaE D Carosella
Sep 19, 2000·American Journal of Obstetrics and Gynecology·J S HuntC Ober
May 30, 2001·Transplantation Proceedings·P MoreauP Paul
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mitsunori ShiroishiKatsumi Maenaka
Sep 10, 2003·Journal of Clinical Immunology·A SteinbornH Grosse-Wilde
Nov 7, 2003·Human Immunology·Magali Le DiscordeEdgardo D Carosella
Aug 12, 2004·Fetal Diagnosis and Therapy·Rinat HackmonYacob Weinstein
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Nathalie Rouas-FreissEdgardo D Carosella
Jul 16, 2005·American Journal of Obstetrics and Gynecology·Shang-mian YieClifford Librach
Dec 22, 2005·PLoS Biology·Sumati RajagopalanEric O Long
May 11, 2006·Human Reproduction·Shang-mian YieClifford L Librach
Jul 11, 2006·Seminars in Fetal & Neonatal Medicine·A M BorzychowskiC W G Redman
Aug 15, 2006·Lancet·Raj Rai, Lesley Regan
Mar 13, 2008·Blood·Edgardo D CarosellaJoel Lemaoult
Apr 4, 2009·American Journal of Reproductive Immunology : AJRI·Roberta RizzoEva Sverremark-Ekström
Jun 17, 2009·American Journal of Reproductive Immunology : AJRI·Ekaterina Ivanova-TodorovaStanimir Kyurkchiev
Mar 25, 2011·Archives of Gynecology and Obstetrics·Ariz AkhterSuraksha Agrawal

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Citations

Aug 22, 2014·Journal of Immunology Research·Vera RebmannPeter A Horn
Feb 6, 2016·American Journal of Reproductive Immunology : AJRI·Arsène MekinianMarie Bornes
Sep 3, 2014·Biomedical Journal·Daniel Vaiman
Apr 8, 2015·Clinical and Experimental Immunology·E FavoinoF Perosa
Oct 24, 2014·Frontiers in Immunology·Erick C CastelliCelso T Mendes-Junior
Nov 14, 2017·American Journal of Reproductive Immunology : AJRI·Bi ShiChristopher J Davies
Jul 20, 2019·HLA : Immune Response Genetics·Moniek H C CraenmehrMichael Eikmans
Apr 24, 2019·Advanced Pharmaceutical Bulletin·Elnaz MosaferiBehzad Baradaran
Aug 1, 2020·Frontiers in Immunology·Giada Amodio, Silvia Gregori
Nov 17, 2020·Expert Review of Clinical Immunology·Sepideh Ebrahimi MeimandNima Rezaei

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