In vitro upconverting/downshifting luminescent detection of tumor markers based on Eu3+ -activated core-shell-shell lanthanide nanoprobes

Chemical Science
Yongsheng LiuXueyuan Chen

Abstract

Trivalent europium (Eu3+) doped inorganic nanoparticles (NPs), emerging as a new class of red luminescent nanoprobes, have shown great promise in bioapplications as diverse as luminescent bioassays and disease theranostics owing to their superior optical properties such as long-lived downshifting luminescence (DSL) and upconverting luminescence (UCL). However, the exploration of Eu3+-doped NPs as red luminescent bioprobes particularly combined with DSL and UCL of Eu3+ hitherto remains untouched. Herein, we report a rational core-shell-shell (CSS) design strategy to construct Eu3+-activated NaGdF4:Yb/Tm@NaGdF4:Eu@NaEuF4 CSS NPs functionalized with efficient UCL and dissolution-enhanced DSL of Eu3+ for in vitro tumor marker detection and tumor-targeted imaging. By utilizing the CSS NPs as red luminescent nanoprobes, we demonstrate the successful UCL and DSL bioassays of a typical hepatic carcinoma biomarker, alpha-fetoprotein (AFP), in human serum samples. The UCL bioassay shows a limit of detection (LOD) of AFP down to 20 pg mL-1 (290 fM), which is the lowest among luminescent bioassays of AFP ever reported, and a 30-fold improvement relative to that of the commercial dissociation-enhanced lanthanide fluoroimmunoassay kit. Meanw...Continue Reading

References

Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J GoodsonS Rosenberg
Jan 29, 1999·Science·R T WeghA Meijerink
Apr 17, 2001·Pharmaceutical Research·V P ShahA Yacobi
Jun 19, 2004·Journal of Cancer Research and Clinical Oncology·Lun-Xiu Qin, Zhao-You Tang
Sep 28, 2006·Journal of the American Chemical Society·Loïc J CharbonnièreHans-Gerd Löhmannsröben
Dec 22, 2009·Chemical Society Reviews·Svetlana V Eliseeva, Jean-Claude G Bünzli
Dec 23, 2009·Combinatorial Chemistry & High Throughput Screening·Zhuo ChenMingdong Huang
Feb 16, 2010·Chemical Reviews·Jean-Claude G Bünzli
Jun 1, 2011·Angewandte Chemie·Markus Haase, Helmut Schäfer
Oct 25, 2011·Nature Materials·Feng WangXiaogang Liu
Apr 18, 2012·Journal of the American Chemical Society·Xue TengLing Huang
Jun 28, 2012·Journal of the American Chemical Society·Noah J J JohnsonFrank C J M van Veggel
Jun 15, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·A A Terentiev, N T Moldogazieva
Sep 2, 2014·Chemical Society Reviews·Xumeng Wu, Weihong Zhu
Sep 10, 2014·Chemical Society Reviews·Ming-Kiu TsangJianhua Hao
Sep 23, 2014·Chemical Society Reviews·Hao DongChun-Hua Yan
Dec 11, 2014·Chemical Reviews·Jing ZhouFuyou Li
Jan 20, 2015·Nature Nanotechnology·Renren DengXiaogang Liu
Feb 20, 2015·Chemical Society Reviews·Xiaowang LiuXiaogang Liu
Aug 20, 2015·Journal of the American Chemical Society·Zhen LiZhihong Liu
Nov 5, 2015·Nature Nanotechnology·Bo ZhouXiaogang Liu

❮ Previous
Next ❯

Citations

Sep 5, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Y N LiX Q Cui
Apr 25, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Nan LiuEva Hemmer
Feb 27, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Qi ZhuFeng Wang
Jun 14, 2018·Physical Chemistry Chemical Physics : PCCP·Liyuan SunJian-Ping Zhang
Nov 11, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Xiaoqian GeLining Sun
May 15, 2018·Analytical Chemistry·Xiang-Yu OuHuang-Hao Yang
Apr 26, 2018·ACS Applied Materials & Interfaces·Yang-Hui LuoBai-Wang Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
transmission electron microscopy
dynamic light scattering

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.