Aug 1, 1977

In vitro uptake of gentamicin by rat renal cortical tissue

Antimicrobial Agents and Chemotherapy
C H HsuJ M Weller

Abstract

The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 +/- 0.04. The uptake of gentamicin was inhibited by 2 x 10(-5) M dinitrophenol (SW/M = 1.03 +/- 0.04) and by anoxia (SW/M = 1.01 +/- 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin.

Mentioned in this Paper

Dinitrophenols
Gentamicins
Gentacycol
Uptake
Protein Phosphorylation
Kidney
p-Aminohippurate
Structure of Cortex of Kidney
Gentamicin Measurement
Tetraethylammonium Chloride

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