In Vitro Uptake of Hydroxyapatite Nanoparticles and Their Effect on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Stem Cells International
Xing YangQingling Feng

Abstract

There have been many applications in biomedical fields based on hydroxyapatite nanoparticles (HA NPs) over the past decades. However, the biocompatibility of HANPs is affected by exposure dose, particle size, and the way of contact with cells. The objective of this study is to investigate the effect of HA NPs with different sizes on osteogenesis using human mesenchymal stem cells (hMSCs). Three different-sized HA NPs (~50, ~100, and ~150 nm, resp.) were synthesized to study the cytotoxicity, cellular uptake, and effect on osteogenic differentiation of hMSCs. The results clearly showed that each size of HA NPs had dose-dependent cytotoxicity on hMSCs. It was found that HA NPs could be uptaken into hMSCs. The osteogenic differentiation of hMSCs was evaluated through alkaline phosphatase (ALP) activity measurement, ALP staining, immunofluorescent staining for osteopontin (OPN), and real-time polymerase chain reaction (RT-PCR) examination. As expected, HA NPs of all sizes could promote the differentiation of hMSCs towards osteoblast lineage. Among the three sizes, smaller-sized HA NPs (~50 and ~100 nm) appeared to be more effective in stimulating osteogenic differentiation of hMSCs.

References

Jan 4, 1998·Journal of Cellular Biochemistry·K K Kaysinger, W K Ramp
Feb 22, 2002·Journal of Biomedical Materials Research·David H KohnPaul H Krebsbach
May 22, 2002·Cellular Signalling·Neus AgellPriam Villalonga
Jul 22, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Masafumi UotaTsuyoshi Kijima
Oct 18, 2005·Journal of Cellular Biochemistry·Majd Zayzafoon
Apr 16, 2009·Nano Letters·D W Boukhvalov, M I Katsnelson
Feb 16, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Mehdi Sadat-ShojaiLeila Nasiri Khanlar
Aug 10, 2010·Advanced Drug Delivery Reviews·Jin XieXiaoyuan Chen
Aug 28, 2012·International Journal of Nanomedicine·Andrea TautzenbergerAnita Ignatius
Apr 6, 2013·Tissue Engineering. Part B, Reviews·Siti Maisurah ZakariaJohn A Jansen
Apr 16, 2013·Acta Biomaterialia·Mehdi Sadat-ShojaiAhmad Jamshidi
May 15, 2013·Advanced Materials·Akhilesh K GaharwarAli Khademhosseini
May 9, 2014·Journal of Biomedical Nanotechnology·Xujie LiuQingling Feng
Dec 3, 2014·Journal of Colloid and Interface Science·Wan-Kyu KoIl Keun Kwon
Dec 23, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Binrui CaoChuanbin Mao
Jan 23, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Xingjie ZanQian Wang
Jul 27, 2011·Science and Technology of Advanced Materials·Yu-Tzu HuangYusuke Yamauchi
Sep 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Xing YangDafna Benayahu
Jun 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhaoming LiuRuikang Tang
Apr 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yao HeJiandong Ding
Oct 28, 2013·Biomaterials Science·Changchun ZhouXingdong Zhang

❮ Previous
Next ❯

Citations

Dec 17, 2019·Journal of Tissue Engineering and Regenerative Medicine·Yangxi LiuHongli Sun
Jun 12, 2019·Biomedical Materials·Vijay Bhooshan KumarAharon Gedanken
Jan 7, 2020·Current Drug Metabolism·Pooja MalaviyaAbhay R Vasavada
Dec 11, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dinesh K PatelKi-Taek Lim
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Naimeh HashemiHossein Naderi-Manesh
Jan 29, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Otto J JuhlHenry J Donahue
Feb 20, 2021·International Journal of Nanomedicine·Salma El-HabashyAmal H El-Kamel
May 1, 2021·Nanomaterials·Rafaela-Maria KavasiMaria Chatzinikolaidou
May 22, 2020·Current Stem Cell Research & Therapy·Tianxu ZhangRonghui Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
dynamic light scattering

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.