In vitro validation of bioluminescent monitoring of disease progression and therapeutic response in leukaemia model animals

European Journal of Nuclear Medicine and Molecular Imaging
Yusuke InoueKuni Ohtomo

Abstract

The application of in vivo bioluminescence imaging to non-invasive, quantitative monitoring of tumour models relies on a positive correlation between the intensity of bioluminescence and the tumour burden. We conducted cell culture studies to investigate the relationship between bioluminescent signal intensity and viable cell numbers in murine leukaemia model cells. Interleukin-3 (IL-3)-dependent murine pro-B cell line Ba/F3 was transduced with firefly luciferase to generate cells expressing luciferase stably under the control of a retroviral long terminal repeat. The luciferase-expressing cells were transduced with p190 BCR-ABL to give factor-independent proliferation. The cells were cultured under various conditions, and bioluminescent signal intensity was compared with viable cell numbers and the cell cycle stage. The Ba/F3 cells showed autonomous growth as well as stable luciferase expression following transduction with both luciferase and p190 BCR-ABL, and in vivo bioluminescence imaging permitted external detection of these cells implanted into mice. The bioluminescence intensities tended to reflect cell proliferation and responses to imatinib in cell culture studies. However, the luminescence per viable cell was influenc...Continue Reading

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Citations

Sep 22, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Yusuke InoueKuni Ohtomo
Dec 20, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Yusuke InoueKuni Ohtomo
Apr 13, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Marleen KeyaertsTony Lahoutte
Sep 26, 2012·International Journal of Oncology·Myoung Woo LeeHong Hoe Koo
Oct 6, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shunsuke KondoChikao Morimoto

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