PMID: 8943051Nov 26, 1996Paper

In vitro V(D)J recombination: signal joint formation

Proceedings of the National Academy of Sciences of the United States of America
P CortesD Baltimore

Abstract

The first step of V(D)J recombination, specific cleavage at the recombination signal sequence (RSS), can be carried out by the recombination activating proteins RAG1 and RAG2. In vivo, the cleaved coding and signal ends must be rejoined to generate functional antigen receptors and maintain chromosomal integrity. We have investigated signal joint formation using deletion and inversion substrates in a cell free system. RAG1 and RAG2 alone or in combination were unable to generate signal joints. However, RAG1 and RAG2 complemented with nuclear extracts were able to recombine an extrachromosomal substrate and form precise signal joints. The in vitro reaction resembled authentic V(D)J recombination in being Ku-antigen-dependent.

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Citations

May 29, 1998·Current Opinion in Immunology·U GrawunderM R Lieber
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jee-Hyun UmJay H Chung
Jan 20, 1999·The Journal of Experimental Medicine·G BainC Murre
Sep 5, 2003·Nucleic Acids Research·Huichen WangGeorge Iliakis
Feb 19, 2008·Nucleic Acids Research·Prafulla RavalPatrick C Swanson
Apr 28, 2001·Evolution & Development·D S Thaler
Aug 1, 2015·Scientific Reports·Francisca F AlmeidaFlorent Ginhoux
Jun 15, 2005·Scandinavian Journal of Immunology·S W YuanG E Wu
Jan 14, 1999·Molecular Cell·E BesmerP Cortes
Jun 18, 1998·Cell·A VillaE Spanopoulou
Apr 7, 2009·Virology·Shui-Mei Wang, Chin-Tien Wang
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Jul 31, 1997·Nature·D A RamsdenM Gellert
Sep 26, 1997·The Journal of Biological Chemistry·G Chu
Sep 5, 2020·The Biochemical Journal·Namrata M NilavarSathees C Raghavan
Oct 16, 1999·European Journal of Biochemistry·P Labhart
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·V AidinisS Santagata
Mar 19, 1999·Molecular and Cellular Biology·P Labhart
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandru OlaruFerenc Livák
Oct 29, 1997·Molecular and Cellular Biology·F Weis-GarciaP Cortes

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