In vitro white spot syndrome virus (WSSV) replication in explants of the heart of freshwater crab, Paratelphusa hydrodomous

Journal of Virological Methods
K S Nathiga NambiA S Sahul Hameed

Abstract

Explants from different organs of freshwater crab, Paratelphusa hydrodomous were prepared to establish an in vitro system for replication of white spot syndrome virus (WSSV) of shrimp. Heart explants were maintained for 53 days without any morphological changes in EX-CELL™ 405 medium with and without serum whereas the explants of eye muscle, gill, shell membrane and appendage muscle died within 15 days of culture period. The heart explants on different days of culture were exposed to WSSV for 10 days to study the viral replication. The infection of WSSV in explants of the heart was confirmed by PCR, RT-PCR, Western blot, histology, immunohistochemistry, bioassay and transmission electron microscopy. The WSSV was quantified by real-time PCR and indirect ELISA. The WSSV inoculum prepared from the heart explants of crab caused significant mortality in Penaeus monodon in challenge experiments and the results indicate that the WSSV which replicated in the heart explants of freshwater crab maintains its infectivity as in marine shrimp. The results indicate that the heart explants of P. hydrodomous would be a good alternative to whole animals for production of WSSV.

References

Nov 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A H Schuurs, B K Van Weemen
Jul 1, 1990·Experimental Cell Research·J Z TongF Alvarez
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R E ParchmentG B Pierce
Dec 1, 1995·In Vitro Cellular & Developmental Biology. Animal·D GhoshA K Dasmahapatra
Jul 27, 2001·Journal of Medical Virology·L GabrielliM P Landini
May 30, 2002·Diseases of Aquatic Organisms·Subramaniyan ManivannanIddya Karunasagar
Jan 23, 2004·Diseases of Aquatic Organisms·A S Sahul HameedK Yoganandhan
Oct 6, 2004·Diseases of Aquatic Organisms·Kanokporn ChayaburakulBoonsirm Withyachumnarnkul

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Citations

Dec 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Vanessa A AnjosMarta M Souza
Aug 24, 2019·Journal of Aquatic Animal Health·Selvaraj SivakumarNatarajan Kalaimani
Aug 14, 2020·MBio·Sailakshmi VelamoorMihnea Bostina

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