In vivo 5-fluorouracil and fluoronucleotide T1 relaxation time measurements using the variable nutation angle method

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Terence P F GadeJason A Koutcher

Abstract

19Fluorine NMRS has the potential to enable noninvasive predictions of tumor response to 5-fluorouracil (5FU) therapy based on tumor pharmacokinetics. Knowledge of the T1's of 5FU and its fluoronucleotide anabolites (FNuc) is required for quantitative spectral analysis and selection of optimal pulse parameters. We used the variable nutation angle (VNA) method to determine T1's of 5FU and FNuc in subcutaneous Walker 256 rat mammary carcinosarcoma tumors transfected with a cytosine deaminase/uracil phosphoribosyltransferase fusion gene. We calibrated in vivo NAs using methoxydifluoroacetate to ensure the accuracy of these measurements. The T1's were calculated based on signal intensities acquired with NAs of 20 degrees, 35 degrees, 45 degrees, 60 degrees, and 75 degrees. The acquisition order of these NAs was shuffled to reduce the effect of signal variations. The determined T1's for 5FU and FNuc (2.3 +/- 0.1 s and 1.3 +/- 0.1 s, respectively) represent the first reported in vivo measurements for these metabolites in tumor.

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Citations

Apr 17, 2008·Cancer Research·Terence P F GadeJuri G Gelovani
Jul 30, 2011·PloS One·Terence P GadePhilipp Mayer-Kuckuk
Sep 26, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Yoshihiro DoiYoko Kanazawa
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Terence P F GadeJason A Koutcher

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