In vivo activation of naive CD4+ T cells in nasal mucosa-associated lymphoid tissue following intranasal immunization with recombinant Streptococcus gordonii.

Infection and Immunity
D MedagliniGianni Pozzi

Abstract

The antigen-specific primary activation of CD4+ T cells was studied in vivo by adoptive transfer of ovalbumin-specific transgenic T cells (KJ1-26+ CD4+) following intranasal immunization with recombinant Streptococcus gordonii. A strain of S. gordonii expressing on its surface a model vaccine antigen fused to the ovalbumin (OVA) peptide from position 323 to 339 was constructed and used to study the OVA-specific T-cell activation in nasal mucosa-associated lymphoid tissue (NALT), lymph nodes, and spleens of mice immunized by the intranasal route. The recombinant strain, but not the wild type, activated the OVA-specific CD4+ T-cell population in the NALT (89% of KJ1-26+ CD4+ T cells) just 3 days following immunization. In the cervical lymph nodes and in the spleen, the percentage of proliferating cells was initially low, but it reached the peak of activation at day 5 (90%). This antigen-specific clonal expansion of KJ1-26+ CD4+ T cells after intranasal immunization was obtained with live and inactivated recombinant bacteria, and it indicates that the NALT is the site of antigen-specific T-cell priming.

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Citations

Nov 26, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Irina L TutykhinaAlexander L Gintsburg
Sep 15, 2010·Journal of Immune Based Therapies and Vaccines·Lindsay J HallGordon Dougan
Dec 19, 2013·PloS One·Elena PettiniDonata Medaglini
May 12, 2009·Methods : a Companion to Methods in Enzymology·Silvia VendettiMaria Teresa De Magistris
May 5, 2009·Methods : a Companion to Methods in Enzymology·Elena PettiniDonata Medaglini
Jun 5, 2007·Expert Review of Vaccines·Maria Leonor Sarno OliveiraPaulo Lee Ho
Sep 9, 2006·Clinical and Vaccine Immunology : CVI·Annalisa CiabattiniDonata Medaglini
Oct 27, 2016·Frontiers in Immunology·Annalisa CiabattiniDonata Medaglini
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kathryn A MatthiasJeffrey N Weiser
Mar 30, 2018·Frontiers in Immunology·Annalisa CiabattiniDonata Medaglini

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