PMID: 12770006May 29, 2003Paper

In vivo activation of pro-form Bombyx cysteine protease (BCP) in silkmoth eggs: localization of yolk proteins and BCP, and acidification of yolk granules

Journal of Insect Physiology
Y YamahamaS Y Takahashi

Abstract

The present study was designed to investigate the process of acidification of yolk granules during embryogenesis. In oocytes of mature Bombyx mori silkmoth, yolk proteins and a cysteine protease (pro-form BCP) were found in yolk granules. BCP was localized in small sized yolk granules (SYG, 3-6 microm in diameter) and yolk proteins in large sized granules (LYG, 6-11 microm in diameter), which might result in a spatial separation of protease and its substrates to avoid unnecessary hydrolysis. The granules were isolated on Percoll density gradient centrifugation. Although separation of LYG and SYG was incomplete, the granules sedimented in different fractions when using unfertilized egg extract, in which LYG was recovered from heavier fractions and BCP from lighter fractions. Acid phosphatase, as well as other lysosomal marker enzymes tested, was recovered from LYG-containing fractions. When extracts were prepared from developing eggs (day 3), some BCP-containing granules co-sedimented with LYG. The inactive pro-form BCP was activated in vivo, in parallel with yolk protein degradation, and as demonstrated previously in vitro under acidic conditions (). These results suggest that acidification occurs in yolk granules during embryo...Continue Reading

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Citations

May 16, 2013·PloS One·Takayuki YamamotoHiroshi Sawada
Jul 16, 2008·Zoological Science·Yumi YamahamaTakahiko Hariyama
Dec 30, 2008·Insect Biochemistry and Molecular Biology·Lucimar S MottaEdnildo A Machado
Jul 27, 2006·Molecular Reproduction and Development·I B RamosE A Machado
Jul 27, 2015·Insect Biochemistry and Molecular Biology·Nidhi SaikhedkarAshok Giri
Sep 6, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Demetrio RaldúaJoan Cerdà
Feb 26, 2021·Insect Science·Xia XuYong-Ping Huang
Dec 1, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hiroshi SawadaTeruhiko Iino
Sep 9, 2008·Journal of Insect Physiology·F M GomesE A Machado

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