PMID: 9545589May 16, 1998Paper

In vivo activation of yeast plasma membrane H+-ATPase by ethanol: effect on the kinetic parameters and involvement of the carboxyl-terminus regulatory domain

Biochimica Et Biophysica Acta
G A Monteiro, Isabel Sá-Correia

Abstract

The in vivo activation of Saccharomyces cerevisiae plasma membrane H+-ATPase by ethanol was observed during ethanol-stressed cultivation or following the rapid incubation of cells with ethanol (6% (v/v)). Ethanol activated both the basal and the glucose-activated forms of the enzyme being the H+-ATPase fully activated by glucose (5% (w/v)) still additionally activable by ethanol. The kinetic parameters of ethanol-activated and non-activated H+-ATPase were calculated based directly on Michaëlis-Menten equation (with MgATP concentrations in the range 0. 16-8.18 mM and 7.5 mM of free Mg2+); the rectangular hyperbolic function was solved using iterative procedures. Ethanol-induced stimulation of plasma membrane H+-ATPase activity was associated to the increase of Vmax whereas the Km for MgATP increased. Results obtained with mutants constructed and used in previous studies envisaging the analysis of the molecular mechanisms underlying plasma membrane ATPase activation by glucose, external acidification and nitrogen starvation, suggested that the carboxyl-terminus (C-terminus) regulatory domain may also be involved in the in vivo activation by ethanol.

References

Mar 1, 1991·Applied and Environmental Microbiology·M F Rosa, I Sá-Correia
Mar 1, 1991·Journal of General Microbiology·C A Viegas, I Sá-Correia
Jul 25, 1990·Biochimica Et Biophysica Acta·R Serrano, F Portillo
Nov 16, 1987·FEBS Letters·P Eraso, C Gancedo

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Citations

Jul 25, 2008·Applied Biochemistry and Biotechnology·Lei ZhaoTianwei Tan
Jul 28, 2009·Applied and Environmental Microbiology·Miguel C TeixeiraIsabel Sá-Correia
Jan 22, 2013·International Journal of Food Microbiology·Malcolm StratfordDavid B Archer
Mar 13, 2014·The FEBS Journal·Femke I C MensonidesM Joost Teixeira de Mattos
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart
Aug 2, 2006·FEMS Yeast Research·Olga Kinclova-ZimmermannovaHana Sychrova
Jan 27, 2017·Frontiers in Plant Science·Yuan SongLizhe An

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