In Vivo Allergen-Activated Eosinophils Promote Collagen I and Fibronectin Gene Expression in Airway Smooth Muscle Cells via TGF-β 1 Signaling Pathway in Asthma

International Journal of Molecular Sciences
Ieva JanulaityteKestutis Malakauskas

Abstract

Eosinophils infiltration and releasing TGF-β1 in the airways has been implicated in the pathogenesis of asthma, especially during acute episodes provoked by an allergen. TGF-β1 is a major mediator involved in pro-inflammatory responses and fibrotic tissue remodeling in asthma. We aimed to evaluate the effect of in vivo allergen-activated eosinophils on the expression of COL1A1 and FN in ASM cells in asthma. A total of 12 allergic asthma patients and 11 healthy subjects were examined. All study subjects underwent bronchial challenge with D. pteronyssinus allergen. Eosinophils from peripheral blood were isolated before and 24 h after the bronchial allergen challenge using high-density centrifugation and magnetic separation. Individual co-cultures of blood eosinophils and immortalized human ASM cells were prepared. The TGF-β1 concentration in culture supernatants was analyzed using ELISA. Gene expression was analyzed using qRT-PCR. Eosinophils integrins were suppressed with linear RGDS peptide before co-culture with ASM cells. Results: The expression of TGF-β1 in asthmatic eosinophils significantly increased over non-activated asthmatic eosinophils after allergen challenge, p < 0.001. The TGF-β1 concentration in culture supernatan...Continue Reading

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Citations

May 24, 2020·Cells·Andrius JanuskeviciusKestutis Malakauskas
Jan 27, 2021·Trends in Molecular Medicine·Katherine E Lothstein, William C Gause
Jun 21, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Ai JinShemin Lu

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Methods Mentioned

BETA
biopsies
density gradient centrifugation
PCR
ELISA

Clinical Trials Mentioned

NCT03388359

Software Mentioned

GraphPad Prism
GraphPad
ADAM
MC

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