In vivo analysis for mechanism of drug action by juxtacellular recording

Nihon yakurigaku zasshi. Folia pharmacologica Japonica
Hiroki Shikanai, Takeshi Izumi

Abstract

Electrophysiological methods are commonly used in neuroscience and pharmacology to reveal the mechanisms of drug action. In vivo analysis of the mechanisms of drug action is a particularly important method in neuropharmacology. Here, we show the juxtacellular recording method to characterize the electrophysiological and neurochemical properties of neurons. Using juxtacellular recording, researchers can record the membrane potential from single neurons, and examine action potential parameters, such as the width and coefficient variance of inter-spike intervals. Additionally, recorded neurons can be labeled using neurobiotin, and neurochemical properties can be revealed by a combination of immunohistochemical staining and in situ hybridization. We introduce an experiment testing the effects of a phosphodiesterase 4 (PDE4) inhibitor on the fronto-striatal circuit using juxtacellular recording. The cerebral cortex-nucleus accumbens (NAcc)-external segment of globus pallidus (GPe)-subthalamic nucleus (STN)-substantia nigra pars reticulata (SNr) pathway is the neurobiological basis of many neuropsychiatric disorders. Several components of this pathway are particularly important for the regulation of motor action and cognitive functio...Continue Reading

References

Apr 7, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolas MalletFrançois Gonon
Apr 4, 2015·ACS Chemical Neuroscience·Julia T BrouardTrevor Sharp

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