In vivo analysis of axonal transport in zebrafish

Methods in Cell Biology
Catherine M Drerup, Alex V Nechiporuk

Abstract

Intracellular transport of proteins and organelles in neurons plays an essential role in nervous system development and maintenance. Axon outgrowth, synapse formation, and synapse function, among other physiological processes, require active transport of these cargos between the neuronal soma and axon terminals. Abnormalities in this axonal transport are associated with a number of neurodevelopmental and neurodegenerative disorders, such as Charcot-Marie-Tooth disease, Alzheimer disease, and amyotrophic lateral sclerosis. Despite its importance for nervous system development and health, methods for visualizing axonal transport in an intact vertebrate have been lacking. Using the advantages of the zebrafish system, we have developed a straightforward approach to visualize axonal transport of various cargos and motor proteins in intact zebrafish embryos and larvae. Here, we describe this approach in detail and discuss how it can be applied to address questions related to cargo-specific transport regulation and its effects on axon morphology and function in the developing and mature nervous system.

Citations

Sep 3, 2019·ELife·Adam TuttleAlex V Nechiporuk
Mar 28, 2017·F1000Research·James N SleighGiampietro Schiavo
Nov 10, 2018·Frontiers in Cell and Developmental Biology·Amrita MandalCatherine M Drerup
Jun 1, 2019·Communications Biology·Juliane BremerMichael Granato
Mar 7, 2019·Frontiers in Cell and Developmental Biology·Valérie BercierCéline Revenu
Nov 2, 2019·Traffic·Sunaina SuranaGiampietro Schiavo
Feb 1, 2020·Communications Biology·Weili TianHernán López-Schier
Jan 12, 2020·Communications Biology·Juliane BremerMichael Granato

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