In vivo analysis of the half-life of the osteoinductive potential of demineralized bone matrix using diffusion chambers

Calcified Tissue International
R Landesman, A H Reddi

Abstract

Subcutaneous implantation of demineralized bone matrix (DBM) from rat initiates a sequence of developmental events that results in endochondral bone formation. This investigation examined the modification of the osteoinductive potential of DBM during the initial stages of this developmental cascade. Diffusion chambers (DC), constructed with filters of known pore size, permitting or excluding cells from entering the chambers, and containing DBM were subcutaneously implanted into Long-Evans male rats for specific time periods (1-7 days). DC were recovered and the osteoinductive potential of the matrix from these chambers was then tested by subcutaneous implantation and assaying the resulting day 11 plaque tissue enzymatically for alkaline phosphatase activity, and histologically for evidence of chondrogenesis and osteogenesis. The possible modification of DBM by local systemic factors (enzymatic degradation) or contact by polymorphonuclear leukocytes (PMNs) was also investigated. We have concluded from this study that the osteoinductive potential of DBM has a half-life of 5-7 days following implantation and although the enzymes collagenase, elastase, and trypsin abolished this activity, pepsin significantly enhanced it. Culture o...Continue Reading

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Citations

Oct 1, 1993·Calcified Tissue International·L J MardenJ O Hollinger
Feb 1, 1997·Mund-, Kiefer- und Gesichtschirurgie : MKG·N R Kübler
Dec 28, 2011·Cell and Tissue Banking·William S PietrzakGeorge Tsiagalis
Nov 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J E FeighanS Stevenson
Sep 10, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jordan M KatzPredrag Bursac
Apr 25, 2012·Clinical Oral Implants Research·Florian G DraenertWilfried Wagner
Nov 1, 1994·The Journal of Bone and Joint Surgery. American Volume·S StevensonA H Reddi
Aug 15, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·H KuwabaraS Ishii
Dec 1, 1996·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·J Hollinger, M E Wong

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