In vivo analysis of the role of atTic20 in protein import into chloroplasts

The Plant Cell
Xuejun ChenDanny J Schnell

Abstract

The import of nucleus-encoded preproteins into plastids requires the coordinated activities of membrane protein complexes that facilitate the translocation of polypeptides across the envelope double membrane. Tic20 was identified previously as a component of the import machinery of the inner envelope membrane by covalent cross-linking studies with trapped preprotein import intermediates. To investigate the role of Tic20 in preprotein import, we altered the expression of the Arabidopsis Tic20 ortholog (atTic20) by antisense expression. Several antisense lines exhibited pronounced chloroplast defects exemplified by pale leaves, reduced accumulation of plastid proteins, and significant growth defects. The severity of the phenotypes correlated directly with the reduction in levels of atTic20 expression. In vitro import studies with plastids isolated from control and antisense plants indicated that the antisense plastids are defective specifically in protein translocation across the inner envelope membrane. These data suggest that Tic20 functions as a component of the protein-conducting channel at the inner envelope membrane.

References

Oct 1, 1995·The Plant Journal : for Cell and Molecular Biology·E C McKinneyR B Meagher
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Kessler, G Blobel
Dec 11, 1997·Biochimica Et Biophysica Acta·M L JohnstonD D Randall
Feb 21, 1998·The EMBO Journal·S C HinnahJ Soll
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ReumannK Keegstra
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Aug 28, 1999·The Journal of Biological Chemistry·P W van den Wijngaard, W J Vredenberg
Dec 10, 1999·Biophysical Journal·P W van den WijngaardW J Vredenberg
Dec 22, 1999·The Journal of Biological Chemistry·T StahlL Heins
Dec 22, 1999·Trends in Cell Biology·M F BauerM Brunner
Jun 10, 2000·European Journal of Biochemistry·P W van den WijngaardW J Vredenberg
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ReinbotheC Reinbothe
Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·M GutensohnU I Flügge
Oct 13, 2000·Biological Chemistry·N SveshnikovaE Schleiff
Apr 12, 2001·Plant Physiology·D Jackson-Constan, K Keegstra
Apr 21, 2001·Cellular and Molecular Life Sciences : CMLS·J BauerF Kessler
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·L M Fitzpatrick, K Keegstra

❮ Previous
Next ❯

Citations

Aug 16, 2008·Plant Molecular Biology·Ewa Firlej-KwokaBettina Bölter
Jan 25, 2005·Plant Biology·T BeckerE Schleiff
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Giel G van DoorenBoris Striepen
Jan 27, 2011·Plant & Cell Physiology·Yoshino HirabayashiMasato Nakai
May 1, 2009·Plant Physiology·Siddhartha DuttaBaishnab C Tripathy
Oct 4, 2011·BMC Plant Biology·Erika Kovács-BogdánBettina Bölter
Mar 3, 2010·Annual Review of Plant Biology·Hsou-min Li, Chi-Chou Chiu
Aug 12, 2005·Molecular Membrane Biology·Sigrun ReumannKenneth Keegstra
May 13, 2004·The Journal of Cell Biology·Matthew D SmithDanny J Schnell
Oct 10, 2002·FEBS Letters·Henrik Aronsson, Paul Jarvis
Sep 26, 2015·The Plant Journal : for Cell and Molecular Biology·Yubing LiDonna E Fernandez
May 5, 2009·Current Opinion in Cell Biology·Felix Kessler, Danny Schnell
Dec 2, 2008·Trends in Plant Science·Jeferson Gross, Debashish Bhattacharya
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·Nancy Rosenbaum Hofmann, Steven M Theg
Jan 22, 2005·The Plant Journal : for Cell and Molecular Biology·Sabina KovachevaPaul Jarvis
Oct 23, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andrzej BodyłJohn W Stiller
Apr 19, 2003·Physiologia Plantarum·Henrik AronssonClas Dahlin
Dec 17, 2008·The New Phytologist·Paul Jarvis
Nov 21, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Debashish BhattacharyaAdrian Reyes-Prieto
Mar 15, 2011·The Plant Journal : for Cell and Molecular Biology·Ali Reza KasmatiPaul Jarvis
Jan 3, 2006·Journal of Plant Physiology·Michael GutensohnRalf Bernd Klösgen
Jun 12, 2012·Biochimica Et Biophysica Acta·Lilach Sheiner, Boris Striepen
Jan 27, 2010·Biochimica Et Biophysica Acta·Erika Kovács-BogdánBettina Bölter
Oct 25, 2011·Plant Signaling & Behavior·Lan-Xin Shi, Steven M Theg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.