In vivo and in vitro alterations in influenza A/H3N2 virus M2 and hemagglutinin genes: effect of passage in MDCK-SIAT1 cells and conventional MDCK cells

Journal of Clinical Microbiology
Danjuan LiHiroshi Suzuki


No mutations were detected in the hemagglutinin gene of influenza A/H3N2 virus isolates from patients undergoing short-term amantadine treatment. However, genetic changes occurred after serial passage in either MDCK or MDCK-SIAT1 cells. Our results showed that only a few mutations were observed in MDCK-SIAT1-passaged isolates in the presence of amantadine.


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Jan 29, 2002·Archives of Virology·E HoffmannD R Perez
Jan 19, 2007·The New England Journal of Medicine·Reiko SaitoHiroshi Suzuki
May 25, 2007·Molecular Biology and Evolution·Lone SimonsenEdward C Holmes

Related Concepts

Hemagglutinin Glycoproteins, Influenza Virus
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M2 protein, Influenza A virus
Madin Darby Canine Kidney Cells
Serial Passage
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