Abstract
Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the extracellular matrix (ECM), such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This and anticancer properties of lysine, proline, arginine, ascorbic acid, and green tea extract (NM) prompted us to investigate the effect of these nutrients in vitro on human fibrosarcoma cells HT-1080 by measuring cell proliferation, modulation of MMP-2 and MMP-9, and invasive potential. In vivo, we studied the growth of human fibrosarcoma HT-1080 cells in athymic nude mice and the expression of MMPs and VEGF. Cell proliferation was evaluated by MTT assay, MMP expression by gelatinase zymography, and invasion through Matrigel and migration by scratch assay. Tumors were excised, weighed, and processed for histology in both the control and nutrient-supplemented groups. Results showed NM inhibited the growth and reduced the size of tumors in nude mice; decreased MMP-9 and VEGF secretion was found in the supplemented group tissues. NM inhibited invasion through Matrigel a...Continue Reading
Citations
Aug 18, 2010·Cancer Metastasis Reviews·Naghma Khan, Hasan Mukhtar
Nov 13, 2008·Cytotechnology·Jun YeSanetaka Shirahata
Jan 22, 2008·Medical Oncology·M W RoomiA Niedzwiecki
Aug 13, 2009·Journal of Translational Medicine·Chang-Hwan YeomSukchan Lee
Jan 19, 2012·Nutrients·Cynthia MannionMarja Verhoef
May 11, 2013·International Journal of Oncology·M Waheed RoomiMatthias Rath
Oct 3, 2013·International Journal of Oncology·M W RoomiA Niedzwiecki
Oct 14, 2008·Genes & Nutrition·Elena DarraHisanori Suzuki
Sep 12, 2007·Archives of Medical Research·Yadollah Shakiba, Ali Mostafaie
Oct 31, 2006·Cancer Letters·Sebastian GüntherMaria Wartenberg
Mar 17, 2007·Pediatric Blood & Cancer·Holger N LodeGerhard Gaedicke
Apr 1, 2007·Cell Adhesion & Migration·Daizo Yoshida, Akira Teramoto
Jul 3, 2008·Journal of Cardiovascular Pharmacology·Vadim IvanovMatthias Rath
Jul 9, 2009·Phytotherapy Research : PTR·Anup KaleMatthias Rath
Aug 2, 2016·Biological & Pharmaceutical Bulletin·JingZhe LiTakashi Sato
Nov 20, 2020·Animals : an Open Access Journal From MDPI·Limin WeiYing Li