In vivo and in vitro comparison of three different allografts vitalized with human mesenchymal stromal cells

Tissue Engineering. Part a
Laura CoquelinHelene Rouard

Abstract

Bone allografts are commonly used by orthopedists to provide a mechanical support and template for cellular colonization and tissue repair. There is an increasing demand for bone graft substitutes that are safe and easy to store but which are equally effective in supporting new bone growth. In this study, we compared three different human bone allografts: (1) the cryopreserved allograft (frozen), (2) the gamma-irradiated and cryopreserved allograft (γ-irradiated), and (3) the solvent dehydrated and γ-irradiated-processed bone allograft (Tutoplast(®) Process Bone [TPB]). Human mesenchymal stromal cells (hMSCs) have the potential to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Our results showed that hMSC seeding efficiency was equivalent among the three bone allografts. However, differences were observed in terms of cell metabolism (viability), osteoblastic gene expression, and in vivo bone formation. Frozen allografts had the higher frequency of new bone formation in vivo (89%). Compared with frozen allografts, we demonstrated that TPB allografts allowed optimal hMSC viability, osteoblastic differentiation, and bone formation to occur in vivo (72%). Further, the frequency of successful bone formation wa...Continue Reading

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Citations

May 7, 2013·Trends in Biotechnology·Eduardo AnituaGorka Orive
Dec 21, 2012·Tissue Engineering. Part B, Reviews·Matthew B Fisher, Robert L Mauck
Dec 11, 2013·International Orthopaedics·Yasuhiro HommaPhilippe Hernigou
Dec 3, 2014·Journal of Dentistry·E P FerrazA L Rosa
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Luciano VidalPierre Layrolle

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Methods Mentioned

BETA
ELISA
scanning electron microscopy
PCR
light microscopy

Software Mentioned

ImageJ
graphpad

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