In vivo and in vitro effect of Acacia nilotica seed proteinase inhibitors on Helicoverpa armigera (Hübner) larvae.

Journal of Biosciences
S Ramesh BabuI M Santha

Abstract

Acacia nilotica proteinase inhibitor (AnPI) was isolated by ammonium sulphate precipitation followed by chromatography on DEAE-Sephadex A-25 and resulted in a purification of 10.68-fold with a 19.5 percentage yield. Electrophoretic analysis of purified AnPI protein resolved into a single band with molecular weight of approximately 18.6+1.00 kDa. AnPI had high stability at different pH values (2.0 to 10.0) except at pH 5.0 and are thermolabile beyond 80 degree C for 10 min. AnPI exhibited effective against total proteolytic activity and trypsin-like activity, but did not show any inhibitory effect on chymotrypsin activity of midgut of Helicoverpa armigera. The inhibition kinetics studies against H. armigera gut trypsin are of non-competitive type. AnPI had low affinity for H. armigera gut trypsin when compared to SBTI. The partially purified and purified PI proteins-incorporated test diets showed significant reduction in mean larval and pupal weight of H. armigera. The results provide important clues in designing strategies by using the proteinase inhibitors (PIs) from the A. nilotica that can be expressed in genetically engineered plants to confer resistance to H. armigera.

References

Dec 1, 1991·Journal of Biochemistry·J Y LinY S Hsieh
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A JongsmaW J Stiekema
Jul 27, 2001·Journal of Insect Physiology·A TelangD E. Wheeler
May 29, 2003·Journal of Insect Physiology·C Bolter, M A. Jongsma
Nov 1, 1961·Archives of Biochemistry and Biophysics·B F ERLANGERW COHEN
Jan 6, 2004·Journal of Protein Chemistry·Soghra Khatun Haq, Rizwan Hasan Khan
Apr 29, 2004·Journal of Agricultural and Food Chemistry·Maria Lígia Rodrigues MacedoJosé Roberto Postali Parra
Feb 18, 2005·Biochimica Et Biophysica Acta·Vaijayanti A TamhaneVidya S Gupta
Jul 11, 2009·Journal of Agricultural and Food Chemistry·Kah Y EeSamson O Agboola
Apr 27, 2010·Journal of Insect Physiology·Vinod D PardeManvendra S Kachole
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·K M DunseM A Anderson
Oct 5, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Frank Odei-AddoRyno Naude

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