In vivo and in vitro effects of homocysteine on Na+, K+-ATPase activity in parietal, prefrontal and cingulate cortex of young rats

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
Cristiane MattéAngela T S Wyse

Abstract

In the present study we determined the effect of chronic administration of homocysteine on Na+,K+-ATPase activity in synaptic membranes from parietal, prefrontal and cingulate cortex of young rats. We also studied the in vitro effect of homocysteine on this enzyme activity and on some oxidative stress parameters, namely thiobarbituric acid-reactive substances (TBA-RS) and total radical-trapping antioxidant potential (TRAP) in the same cerebral structures. For the in vivo studies, we induced elevated levels of homocysteine in blood (500 microM), comparable to those of human homocystinuria, and in brain (60 nmol/g wet tissue) of young rats by injecting subcutaneously homocysteine (0.3-0.6 micromol/g of body weight) twice a day at 8 h intervals from the 6th to the 28th postpartum day. Controls received saline in the same volumes. Rats were killed 12 h after the last injection. Chronic administration of homocysteine significantly decreased (50%) Na+,K+-ATPase activity in parietal, increased (36%) in prefrontal and did not alter in cingulate cortex of young rats. In vitro homocysteine decreased Na+,K+-ATPase activity and TRAP and increased TBA-RS in all cerebral structures studied. It is proposed that the alteration of Na+,K+-ATPase...Continue Reading

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Citations

Aug 29, 2009·Cellular and Molecular Neurobiology·Dragan HrncićOlivera Stanojlović
Oct 4, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Charlotte E TeunissenHenk J Blom
Sep 29, 2011·Molecular and Cellular Biochemistry·Aline A da CunhaAngela T S Wyse
Nov 26, 2009·Cellular and Molecular Neurobiology·Samanta Oliveira LoureiroRegina Pessoa-Pureur
Jun 28, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Emilene B S SchererAngela T S Wyse
Nov 17, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Cristiane MattéAngela T S Wyse
Oct 14, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Samanta Oliveira LoureiroRegina Pessoa-Pureur
Apr 15, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Samanta Oliveira LoureiroRegina Pessoa-Pureur
Nov 21, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Cristiane MattéAngela T S Wyse
May 8, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Emilene B S SchererAngela T S Wyse
Aug 26, 2006·Neuroscience Letters·Fernanda BonattoJosé Cláudio Fonseca Moreira
Mar 18, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Bárbara TagliariAngela T S Wyse
Jan 31, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Cristiane MattéAngela T S Wyse
Oct 27, 2007·Stroke; a Journal of Cerebral Circulation·Michael A Moskowitz, Tobias Kurth
Jun 9, 2005·Metabolic Brain Disease·Francieli M StefanelloAngela T S Wyse
Mar 18, 2008·Neurochemical Research·Alexandra I ZugnoAngela T S Wyse
Apr 29, 2010·Metabolic Brain Disease·Aline A da CunhaAngela T S Wyse
Jul 1, 2011·Molecular and Cellular Biochemistry·Aline A da CunhaAngela T S Wyse
Oct 22, 2016·Amino Acids·Bruna M Schweinberger, Angela T S Wyse
Nov 2, 2016·Journal of Alzheimer's Disease : JAD·Martina PařízkováJan Laczó

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