Abstract
This study aimed to investigate the effect of parathyroid hormone (PTH1-34) on osteogenic and adipogenic differentiation of hBMSCs by the regulation of miR-155. A total of 36 adult volunteers were selected in this study. Effects of PTH1-34 on the proliferation of hBMSCs and miR-155 expression were investigated using a MTT assay. The hBMSCs were divided into blank, PTH1-34, miR-155 mimic, miR-155 mimic negative control (NC), miR-155 inhibitor, miR-155 inhibitor NC, PTH1-34 + miR-155 mimic, PTH1-34 + miR-155 inhibitor, and NPS R-568 groups. Postmenopausal osteoporosis (PMOP) mouse models were established by ovariectomy (OVX) and divided into ten groups. The RT-qPCR and Western blotting assay were carried out to detect the expression of osteogenesis/adipogenic-related genes and miR-155, and osteogenesis/adipogenic-related proteins. PTH1-34 could promote hBMSCs proliferation and inhibit miR-155 expression in a dose-dependent manner. Compared with the blank control group, expressions of Runx2, and BSP was up-regulated in the PTH1-34 and miR-155 inhibitor groups, while expressions of miR-155, PPAR-γ, lipoprotein lipase (LPL), fatty acid binding protein 4 (Fabp4), adiponectin, and CCAAT/enhancer binding protein α (C/EBPα) were down-re...Continue Reading
References
Jan 5, 2000·Kidney International·M WadaJ Fox
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Wei HuangYi-Ping Li
Dec 3, 2010·The Journal of Biological Chemistry·Julie M QuachT John Martin
Feb 16, 2011·Arthritis and Rheumatism·Stephan BlümlKurt Redlich
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tilde EskildsenMoustapha Kassem
Jul 12, 2011·RNA Biology·Jin-fang ZhangHsiang-fu Kung
Nov 25, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Socrates PapapoulosHenry G Bone
Nov 26, 2011·Archives of Gynecology and Obstetrics·Ae Ra KangJong Soon Choi
Dec 16, 2011·Genes, Chromosomes & Cancer·Yuhsin V WuSamuel Singer
Jan 20, 2012·Cardiovascular Research·Philipp DiehlKarlheinz Peter
May 31, 2012·Perspectives in Biology and Medicine·Kathleen K Eggleson
Dec 19, 2012·Gene·Terry S EltonNarasimham L Parinandi
Apr 23, 2013·Current Osteoporosis Reports·Andre J van WijnenSanjeev Kakar
May 7, 2013·Bone·Stephen Y KangSteven R Buchman
Sep 12, 2013·PloS One·Qing WangLei Chen
Jan 18, 2014·Journal of Cellular Physiology·S VimalrajN Selvamurugan
Feb 8, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Fen DuDaping Fan
Mar 22, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qing HuJian-hua Zhao
Apr 23, 2014·Molecular and Cellular Biochemistry·Jin-Zhu FanJian Liu
Oct 25, 2014·BMB Reports·Hara Kang, Akiko Hata
Nov 27, 2014·Journal of Cellular Biochemistry·Abdul S QadirJeong-Hwa Baek
Dec 17, 2014·Biological Chemistry·Xiao-Dong LiuAn-Li Yang
Jan 10, 2016·Bone·Navya LaxmanAndreas Kindmark
Jun 11, 2016·Experimental and Therapeutic Medicine·Bailing ChenHaowen Cui
Jun 28, 2016·Experimental and Therapeutic Medicine·DA-An ZhouJian Jun Li
Jun 29, 2016·Journal of Oral Science·Yoshifumi OkiKazuhiro Tsuga
Jul 15, 2016·Rheumatology·Aziza ElmesmariMariola Kurowska-Stolarska
Jul 19, 2016·Cell and Tissue Research·Cong HuangSiwen Jiang
Aug 25, 2016·International Journal of Molecular Sciences·Xiaochen QuYan Zeng
Oct 16, 2016·Archives of Osteoporosis·K L BarkerC J Minns Lowe
Oct 21, 2016·PloS One·Hai-Juan LiuXiao-Fei Yang
Dec 3, 2016·Scientific Reports·Chenxi GuGuoqiang Zhao
Citations
Jan 16, 2019·American Journal of Physiology. Endocrinology and Metabolism·Yu ShaoJoseph P Bidwell
Dec 31, 2019·Journal of Cellular Biochemistry·Mingwei HuangHongcheng Ding
May 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhonglong LiuYue He
Sep 19, 2019·Biotechnology and Bioengineering·Mikhael Hadida, David Marchat
Sep 1, 2018·Stem Cell Research & Therapy·Yangling WangLingling Cao