In vivo and in vitro effects of PTH1-34 on osteogenic and adipogenic differentiation of human bone marrow-derived mesenchymal stem cells through regulating microRNA-155

Journal of Cellular Biochemistry
Huan-Sheng HanShuo Geng

Abstract

This study aimed to investigate the effect of parathyroid hormone (PTH1-34) on osteogenic and adipogenic differentiation of hBMSCs by the regulation of miR-155. A total of 36 adult volunteers were selected in this study. Effects of PTH1-34 on the proliferation of hBMSCs and miR-155 expression were investigated using a MTT assay. The hBMSCs were divided into blank, PTH1-34, miR-155 mimic, miR-155 mimic negative control (NC), miR-155 inhibitor, miR-155 inhibitor NC, PTH1-34 + miR-155 mimic, PTH1-34 + miR-155 inhibitor, and NPS R-568 groups. Postmenopausal osteoporosis (PMOP) mouse models were established by ovariectomy (OVX) and divided into ten groups. The RT-qPCR and Western blotting assay were carried out to detect the expression of osteogenesis/adipogenic-related genes and miR-155, and osteogenesis/adipogenic-related proteins. PTH1-34 could promote hBMSCs proliferation and inhibit miR-155 expression in a dose-dependent manner. Compared with the blank control group, expressions of Runx2, and BSP was up-regulated in the PTH1-34 and miR-155 inhibitor groups, while expressions of miR-155, PPAR-γ, lipoprotein lipase (LPL), fatty acid binding protein 4 (Fabp4), adiponectin, and CCAAT/enhancer binding protein α (C/EBPα) were down-re...Continue Reading

References

May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Wei HuangYi-Ping Li
Dec 3, 2010·The Journal of Biological Chemistry·Julie M QuachT John Martin
Feb 16, 2011·Arthritis and Rheumatism·Stephan BlümlKurt Redlich
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tilde EskildsenMoustapha Kassem
Nov 25, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Socrates PapapoulosHenry G Bone
Dec 16, 2011·Genes, Chromosomes & Cancer·Yuhsin V WuSamuel Singer
Jan 20, 2012·Cardiovascular Research·Philipp DiehlKarlheinz Peter
May 31, 2012·Perspectives in Biology and Medicine·Kathleen K Eggleson
Dec 19, 2012·Gene·Terry S EltonNarasimham L Parinandi
Apr 23, 2013·Current Osteoporosis Reports·Andre J van WijnenSanjeev Kakar
Jan 18, 2014·Journal of Cellular Physiology·S VimalrajN Selvamurugan
Mar 22, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qing HuJian-hua Zhao
Nov 27, 2014·Journal of Cellular Biochemistry·Abdul S QadirJeong-Hwa Baek
Jul 15, 2016·Rheumatology·Aziza ElmesmariMariola Kurowska-Stolarska
Jul 19, 2016·Cell and Tissue Research·Cong HuangSiwen Jiang
Oct 16, 2016·Archives of Osteoporosis·K L BarkerC J Minns Lowe

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Citations

Jan 16, 2019·American Journal of Physiology. Endocrinology and Metabolism·Yu ShaoJoseph P Bidwell
Dec 31, 2019·Journal of Cellular Biochemistry·Mingwei HuangHongcheng Ding
May 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhonglong LiuYue He
Sep 19, 2019·Biotechnology and Bioengineering·Mikhael Hadida, David Marchat

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