In vivo and in vitro kinetics of nitrogenase.

Journal of Bacteriology
L C Davis, Y L Wang

Abstract

We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide was a noncompetitive inhibitor of acetylene reduction; azide was a noncompetitive inhibitor of acetylene reduction, and nitrogen was a partial inhibitor of acetylene reduction. Cyanide was a noncompetitive inhibitor of acetylene reduction in C. pasteurianum but it was a metabolic poison in K. pneumoniae, in addition to being an inhibitor of nitrogenase. The partial nature of nitrogen inhibition was apparent in assays where both nitrogen and CO were present. Nitrogen did not alter the apparent Ki for CO, nor did the presence of CO enhance the competitive effectiveness of nitrogen. By using recombined nitrogenase fractions, we found that the ability of nitrogen to inhibit hydrogen evolution or acetylene reduction varied with the ratio of protein components. The in vivo inhibition of acetylene reduction by dinitrogen was comparable to that obtained with an excess of the Fe protein in vitro. We conclude that there is an...Continue Reading

References

Aug 1, 1975·Journal of Bacteriology·J M Rivera-Ortiz, R H Burris
Jan 1, 1972·Methods in Enzymology·W A Bulen, J R LeComte
Jan 18, 1973·Biochimica Et Biophysica Acta·J C HwangR H Burris
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R A Parejko, P W Wilson
Nov 15, 1965·Biochimica Et Biophysica Acta·A Lockshin, R H Burris
Jan 1, 1958·Journal of Bacteriology·R M PENGRA, P W WILSON
Dec 1, 1959·Canadian Journal of Microbiology·D W WESTLAKE, P W WILSON
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K R Schubert, H J Evans

❮ Previous
Next ❯

Citations

Oct 1, 1984·Archives of Microbiology·M A MurryJ D Tjepkema
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Jason ChristiansenLance C Seefeldt
Feb 1, 1987·Biotechnology and Bioengineering·G T JonesL E Erickson
Mar 6, 2021·The Journal of Biological Chemistry·Michael S MedinaCedric P Owens
Jun 17, 2020·Chemical Reviews·Oliver Einsle, Douglas C Rees
Nov 1, 1986·Applied and Environmental Microbiology·J E UrbanS J Brown
Jun 21, 2005·Inorganic Chemistry·Johannes Kästner, Peter E Blöchl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.