PMID: 8594880Dec 11, 1995Paper

In vivo and in vitro osmotic regulation of HSP-70 and prostaglandin synthase gene expression in kidney cells

The American Journal of Physiology
B D CowleyW Wilkins

Abstract

As a function of the urinary concentrating mechanism, the cells of the renal medulla are exposed to elevated and constantly varying osmolalities and adapt to this environment by selectively expressing certain mRNAs. We evaluated the expression and regulation of two RNAs that may be important in adaptation of rental medullary cells to hyperosmolality. We demonstrate selective, modulated expression in the renal medulla of heat shock protein HSP-70 mRNA and prostaglandin synthase-1 mRNA, with the abundance of these two mRNAs regulated in vivo in concert with changes in medullary sodium and urea. We also determined the abundance of these mRNAs in cultured kidney cells (MDCK) in response to an increase in extracellular osmolality due to selected osmotic agents. HSP-70 and prostaglandin synthase-2 mRNA levels increased when extracellular osmolality was increased to 400-600 mosmol/kg by the addition of NaCl. At 500 mosmol/kg this response was evident at 6 h, was maximal near 24 h, and persisted for a total of 90 days. Prostaglandin synthase-1 mRNA levels in MDCK cells were also increased after chronic exposure to extracellular osmolality. Increased extracellular osmolality caused by agents to which cells are impermeable caused increas...Continue Reading

Citations

Feb 16, 2005·Annual Review of Physiology·Wolfgang Neuhofer, Franz-X Beck
Jan 18, 2008·American Journal of Physiology. Renal Physiology·Olga SazonovaGraham F Wagner
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Udo Hasler
Aug 5, 2000·American Journal of Physiology. Renal Physiology·F X BeckE Müller
Oct 19, 2007·American Journal of Physiology. Cell Physiology·Christoph KüperWolfgang Neuhofer
Nov 14, 2008·American Journal of Physiology. Cell Physiology·Daniela SteinertWolfgang Neuhofer
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva

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