In vivo and in vitro radiotherapy increased dentin enzymatic activity.

Journal of Dentistry
Sandra Ribeiro CunhaL Breschi

Abstract

The present study investigated the effects of in vitro and in-vivo radiotherapy on endogenous enzymatic activity in dentin using gelatin zymography and in-situ zymography. Gelatin zymographic assays were performed on protein extracts obtained from dentin powder of sound non-irradiated (NRT), in vitro irradiated (VTRT) and in vivo irradiated (VIRT) human teeth. Their proteolytic activities were quantified using band densitometric evaluation. For in-situ zymography, dentin specimens from NRT, VIRT and VTRT were covered with fluorescein-conjugated gelatin and examined with confocal laser-scanning microscopy. Fluorescence intensity emitted by the hydrolyzed fluorescein-conjugated gelatin was quantified and statistically analyzed. In-situ zymography data were statistically analyzed using Kruskal-Wallis ANOVA and Dunn's multiple comparison procedures (α = 0.05). No difference between in vitro and in vivo radiotherapy treatment was found. Both VTRT and VIRT groups showed increase in MMP-9 expression when compared to NRT group. Significant increases (p < 0.05) in gelatinolytic activity (26 % for VTRT; 55 % for VIRT) were observed when compared to the NRT group. Radiotherapy increase endogenous enzymatic activity in non-restored dentin.

References

Feb 1, 1977·Journal of Dental Research·S DreizenJ B Drane
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H E Van Wart, H Birkedal-Hansen
Jan 1, 1997·Free Radical Biology & Medicine·P BrenneisenK Scharffetter-Kochanek
Jan 1, 1997·Journal of Dental Research·K Kawasaki, J D Featherstone
Jan 1, 1997·Caries Research·A J van StrijpJ M ten Cate
Sep 1, 1999·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·S Silverman
Aug 14, 2001·Journal of Dental Research·M SulkalaL Tjäderhane
Nov 19, 2003·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Feb 26, 2004·Journal of Dental Research·D H PashleyS Ito
Dec 24, 2005·Scandinavian Journal of Gastroenterology·Carine Strup-PerrotDenis Mathe
Apr 1, 2006·The Lancet Oncology·Andrej M KielbassaHendrik Meyer-Lückel
Apr 25, 2007·Journal of Dental Research·A MazzoniL Breschi
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 7, 2009·Journal of Dental Research·G V MacedoA K Bedran-Russo
Feb 23, 2012·Journal of Dental Research·A MazzoniL Breschi
Mar 21, 2012·International Journal of Radiation Oncology, Biology, Physics·Won Hee LeeYong Woo Lee
Jul 23, 2013·Clinical Oral Investigations·H F J Lieshout, C P Bots
Dec 18, 2013·Journal of Dental Research·A MazzoniL Breschi
Dec 21, 2013·Journal of Dental Research·C M P VidalM R Carrilho
Mar 13, 2014·Journal of Dentistry·J D McGuireJ P Gorski
May 17, 2014·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Michael B BartonGeoff P Delaney
Sep 13, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Abdul R Al-AzriRichard M Logan
Dec 24, 2014·Journal of Dental Research·A MazzoniL Breschi
Jul 23, 2015·Oral Oncology·Jie DengBarbara A Murphy
Sep 10, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Vincent GrégoireSandra Nuyts
Apr 19, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Viviane HassAlessandra Reis
Jan 26, 2017·Caries Research·Cristhian C MadridMario Fernando De Goes
Apr 18, 2017·Archives of Oral Biology·Sandra Ribeiro de Barros da CunhaAna Cecília Corrêa Aranha
Jun 13, 2017·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Wagner Gomes-SilvaAlan Roger Santos-Silva
Sep 27, 2018·Caries Research·Alexandra Mussolino QueirozFrancisco Wanderley Garcia Paula-Silva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Voice : Official Journal of the Voice Foundation
Janine Santos RamosKelly Cristina Alves Silverio
Dental Materials : Official Publication of the Academy of Dental Materials
R Seseogullari-DirihanArzu Tezvergil-Mutluay
Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco
Katharine BowkerSue Cooper
© 2021 Meta ULC. All rights reserved