PMID: 3746294Aug 1, 1986Paper

In vivo and in vitro studies of hafnium-binding to rat serum transferrin

Journal of Inorganic Biochemistry
G M ThenW G Thies

Abstract

The binding of hafnium to rat serum transferrin was studied using the time differential perturbed angular correlation (TDPAC) technique. Hafnium is interesting as a toxic metal binding to transferrin because it behaves metabolically similarly to plutonium. The isotope 181Hf offers favorable access to the TDPAC-method. Samples were prepared in vivo by intravenous injection of Hf-NTA, Hf-citrate, and Hf-oxalate solutions, respectively, into Sprague-Dawley rats and in vitro by adding Hf-NTA solution to fresh rat serum. In both cases two specific electric quadrupole interactions were observed, which correspond to two well-defined binding configurations. They may be attributed to the N-terminal and the C-terminal binding site in the transferrin molecule. The 181Hf-distribution between these two binding states depends on pH, salt and hafnium concentrations, temperature, and incubation time. With a fast TDPAC-setup of four BaF2-detectors a time resolution of about 600 ps could be achieved. The specific binding configurations of 181Hf and the comparatively slow relaxation times lead to spectra of considerable accuracy.

References

Jul 1, 1985·The British Journal of Radiology·D M Taylor, F Planas-Bohne
Jun 1, 1984·The International Journal of Applied Radiation and Isotopes·F A SmithS L Waters
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R T MacGillivrayK Brew
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Citations

Jan 1, 1989·International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes·P J MarsdenR W Evans
Jan 1, 1991·International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes·R BauerM Zeppezauer
Jun 16, 2000·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·S LahirN R Das
May 7, 2010·Bioinorganic Chemistry and Applications·Yanbo ShiPeter J Sadler
Feb 25, 2003·Nature Structural Biology·Dmitriy AlexeevPeter J Sadler
Sep 9, 2004·Chemical Reviews·Lars HemmingsenEva Danielsen

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