In vivo anticlastogenic effect of silymarin from milk thistle Silybum marianum L

Indian Journal of Pharmacology
Sirajudheen AnwarMohamed E Wagih

Abstract

Silymarin, extracted from the seeds of Silybum marianum L. (Milk thistle), is traditionally used for treating various illnesses such as diabetes, cancer, inflammation, hepatitis, liver cirrhosis, and renal problems. Acute cytotoxicity and genotoxicity studies have been reported with ambiguous outcomes; however, its relevant anticlastogenic potential is not yet evaluated. This study was aimed to evaluate in vivo subacute anticlastogenic properties of silymarin to validate its use as a medicinal agent. Silymarin was isolated from seeds of milk thistle. Various genotoxicity bioassays of silymarin were performed using mice. First, the bone marrow cell proliferation was estimated by calculating mitotic index. Second, the chromosomal abnormalities in mice bone marrow cells were studied. Third, micronucleated polychromatic erythrocytes (MPE) test and in vivo activation of sister chromatid exchanges (SCEs) were carried out in mice bone marrow cells. Finally, primary spermatocytes were analyzed to estimate genotoxic effect of silymarin on germ cells. We found that silymarin is capable of inducing a significant increase (P ≤ 0.05) in cell proliferation of bone marrow cells. There is no increase in chromosomal aberrations following silyma...Continue Reading

Citations

May 10, 2019·Phytotherapy Research : PTR·Vahid SoleimaniGholamreza Karimi
Mar 20, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sirajudheen AnwarKannan Rr Rengasamy

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BETA
chromosomal aberrations

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SPSS®

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