PMID: 8601334Mar 1, 1996Paper

In vivo assay for protein-protein interactions using Drosophila chromosomes

Chromosoma
J S PlateroJ C Eissenberg

Abstract

The ability of a chimeric HP1-Polycomb (Pc) protein to bind both to heterochromatin and to euchromatic sites of Pc protein binding was exploited to detect stable protein-protein interactions in vivo. Previously, we showed that endogenous Pc protein was recruited to ectopic heterochromatic binding sites by the chimeric protein. Here, we examine the association of other Pc group (Pc-G) proteins. We show that Posterior sex combs (Psc) protein also is recruited to heterochromatin by the chimeric protein, demonstrating that Psc protein participates in direct protein-protein interaction with Pc protein or Pc-associated protein. In flies carrying temperature-sensitive alleles of Enhancer of zeste[E(z)] the general decondensation of polytene chromosomes that occurs at the restrictive temperature is associated with loss of binding of endogenous Pc and chimeric HP1-Polycomb protein to euchromatin, but binding of HP1 and chimeric HP1-Polycomb protein to the heterochromatin is maintained. The E(z) mutation also results in the loss of chimera-dependent binding to heterochromatin by endogenous Pc and Psc proteins at the restrictive temperature, suggesting that interaction of these proteins is mediated by E(z) protein. A myc-tagged full-lengt...Continue Reading

References

Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Paro, D S Hogness
Dec 1, 1990·Trends in Genetics : TIG·R Paro
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A M MazoI B Dawid
Jan 1, 1989·Developmental Genetics·P N AdlerB Brunk
Sep 1, 1989·Molecular & General Genetics : MGG·C T WuW M Gelbart
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B A Mozer, I B Dawid

❮ Previous
Next ❯

Citations

Jul 5, 2003·Experimental Hematology·Julie Lessard, Guy Sauvageau
Jul 31, 2001·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·S Netter, A Boivin
Jun 26, 1998·Current Opinion in Cell Biology·G Cavalli, R Paro
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·N J Francis, R E Kingston
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeshi AkasakaHisashi Koga
Apr 8, 2000·Current Opinion in Genetics & Development·J C Eissenberg, S C Elgin
Nov 30, 2005·Molecular and Cellular Biology·Inmaculada Hernández-MuñozMaarten van Lohuizen
Nov 15, 2006·Molecular and Cellular Biology·Anton GolovninPavel Georgiev
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·Y ZhangR S Jones
Mar 29, 2008·EMBO Reports·Anton GolovninPavel Georgiev
Sep 19, 1998·Molecular and Cellular Biology·O DenisenkoK Bomsztyk
Nov 24, 2007·The EMBO Journal·Maria M KurshakovaSofia G Georgieva
Apr 17, 1999·Molecular and Cellular Biology·A SoldatovP Georgiev
Dec 1, 2004·Annual Review of Genetics·Leonie Ringrose, Renato Paro
Apr 1, 1997·Current Opinion in Genetics & Development·V Pirrotta

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.