PMID: 7030392Oct 27, 1981Paper

In vivo assembly of newly synthesized histones

Biochemistry
R L Seale

Abstract

Following a labeling period of 2 min, HeLa histones continue to accumulate in chromatin for 10 min, indicating the presence of a histone pool. During the accumulation period, H2A and H2B enter chromatin immediately, while entry of H3 and H4 is more prolonged. Association of newly synthesized core histones with chromatin does not necessarily indicate assembly. When 2-min [3H]lysine-labeled chromatin is exposed to 0.45 M NaCl, nearly half of the newly synthesized histones are dissociated, while mature core histones are stable. H2A and 70% of H2B are salt stable and remain with newly synthesized polynucleosomes. About 30% of H2B, 50% of H4, and all of H3 are salt labile; thus, both the new nucleosomal core histones and salt-labile new core histones are nonstoichiometric. Pulse-labeled core histones are more trypsin-sensitive than mature histones. When the salt-labile, newly synthesized histones are removed, the remaining proteins have the same trypsin sensitivity as bulk core protein. Examination of the tryptic peptides indicated that the increased trypsin sensitivity was due to complete destruction of the loosely associated core histones which undergo a lag prior to assembly. The altered order of appearance of two peptides in str...Continue Reading

References

Nov 1, 1978·Cell·A WorcelM L Wong
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E GermondD Brutlag
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R L Seale
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Weisbrod, H Weintraub
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A Ruiz-CarrilloR Lurz
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A SteinM Bina
Oct 10, 1979·Nucleic Acids Research·H Weintraub
May 1, 1977·Developmental Biology·H R Woodland, E D Adamson
Jun 25, 1976·Journal of Molecular Biology·V JacksonR Chalkley
Oct 10, 1975·Science·A Ruiz-CarrilloV G Allfrey
May 25, 1975·Journal of Molecular Biology·R L Seale, R T Simpson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Weintraub, F Van Lente
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Feb 27, 1980·Biochemical and Biophysical Research Communications·C Crémisi, M Yaniv
Aug 21, 1980·Nature·R A Laskey, W C Earnshaw
Jan 1, 1980·Annual Review of Biochemistry·M L DePamphilis, P M Wassarman
Jan 1, 1980·Progress in Nucleic Acid Research and Molecular Biology·D MathisP Chambon
Jul 10, 1981·Nucleic Acids Research·R L Seale
Mar 15, 1980·Developmental Biology·R J ShihK Keem

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Citations

Jan 1, 1983·Molecular and Cellular Biochemistry·A T Annunziato, R L Seale
Jan 15, 1985·European Journal of Biochemistry·T SenshuK Takahashi
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BavykinA Mirzabekov
Jan 1, 1986·CRC Critical Reviews in Biochemistry·R S WuW M Bonner
Mar 15, 1983·Biochemistry·J H Waterborg, H R Matthews
Jun 18, 1985·Biochemistry·L Louters, R Chalkley
Feb 9, 2006·The Journal of Biological Chemistry·Laura J BensonAnthony T Annunziato
Feb 9, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Laure J M JasonJuan Ausió
Aug 1, 1983·Journal of Virology·M J Fedor, E Daniell

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