In vivo Bcl-2 oncogene neuronal expression in the rat spinal cord

Spine
Jueren LouM O'Brien

Abstract

An acute mechanical rat spinal cord injury model was used to investigate in vivo Bcl-2 oncogene overexpression in neuronal tissue. To introduce the Bcl-2 oncogene in vivo by a recombinant adenovirus vector into rat spinal cord tissue, and to investigate any potential protective effect on neural tissue in the zone of injury in a rat spinal cord model. The Bcl-2 oncogene inhibits apoptotic and necrotic neural cell death in vitro by regulating an antioxidant pathway at sites of free radical generation. Thus, overexpression of the Bcl-2 oncogene may have a role in limiting the secondary injury cascade of spinal cord injury through its regulation of antioxidants. After confirmation of Bcl-2 gene expression in vitro and in vivo in the rat spinal cord, a weight-drop spinal cord injury model was performed on seven rats with prior Bcl-2 inoculation, and on seven rats with prior B-gal inoculation (controls). In vivo Bcl-2 expression was documented by immunostaining. After spinal cord harvest, quantification of percentage preserved tissue at the spinal cord injury site suggested that Bcl-2 overexpression confers neuroprotection. In vivo Bcl-2 oncogene overexpression was successfully induced in neuronal tissue. After Bcl-2 oncogene express...Continue Reading

References

Jan 11, 1992·Journal of Neurotrauma·K B QuattrocchiF C Wagner
Jan 1, 1992·Journal of Neurotrauma·S S PanterE D Hall
Jan 1, 1992·Journal of Neurotrauma·D W Choi
Jan 1, 1992·Journal of Neurotrauma·A R Blight
Aug 1, 1992·Immunology Today·S J Korsmeyer
Jan 1, 1989·Spine·L Janssen, R R Hansebout
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J L ZweierG A Lutty
Jan 1, 1988·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A J BanesL Dahners
Jan 1, 1986·Central Nervous System Trauma : Journal of the American Paralysis Association·W F CollinsE Ogle
Apr 1, 1985·Experimental Neurology·J R WrathallF Harvey
Feb 1, 1985·Annals of Plastic Surgery·K L JonesJ C Fisher
Nov 1, 1985·The Journal of Clinical Investigation·E Braunwald, R A Kloner
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Apr 1, 1993·Current Opinion in Cell Biology·S LeeM B Small
Sep 1, 1992·Trends in Cell Biology·G T WilliamsE A Grimes
Dec 1, 1991·Current Biology : CB·C I Bargmann

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Citations

Feb 11, 1999·Journal of Neuroscience Research·G M Smith, M I Romero
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Dec 19, 1998·Molecular Medicine Today·M J During, L M Ashenden
Nov 28, 2001·Journal of Neurotrauma·J QiuJ R Perez-Polo
Dec 17, 2002·Journal of Neurotrauma·Yasutsugu YukawaLawrence G Lenke
Jun 26, 2003·Neurosurgery·Toshitaka SekiYoshinobu Iwasaki
Jan 4, 2003·Nature Reviews. Neuroscience·Robert M Sapolsky

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