In vivo behaviour of long-circulating liposomes in blood vessels in hamster inflammation and septic shock models-use of intravital fluorescence microscopy

Luminescence : the Journal of Biological and Chemical Luminescence
J M DevoisselleSerge Mordon

Abstract

This study aimed to observe liposome uptake by leukocytes in vivo. The study was performed on skin by using a dorsal skin-fold chamber implanted in golden hamsters using intravital microscopy. 5 and 6-CF-encapsulated polyethylene glycolated liposomes were injected intravenously. The skin microcirculation was observed with an intravital Eclipse E800 Nikon microscope (using x40, x80 magnification) fitted with a Xenon light source and an epifluorescence assembly (excitation, 470 nm, FWHM 40 nm; emission, 540 nm, FWHM 40 nm). An ultra-high sensitivity videocamera mounted on the microscope projected the image onto a monitor, and the images (720 x 576 pixels) were recorded for playback analysis with a digital video cassette recorder. An acute inflammatory response was obtained by removing one complete layer of skin and the underlying fascia and avascular tissue on the opposing side of the flap corresponding to an area equivalent to the window aperture. Using this model and set-up, leukocyte rolling and adhesion were easily observed and the entry of PEGylated liposomes into hamster blood leukocytes was studied for a period of 6 h. PEGylated liposomes were clearly identified alone inside the blood flow and inside the leukocytes as soon...Continue Reading

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R Mauk, R C Gamble
Aug 14, 1992·Biochimica Et Biophysica Acta·M C Woodle, D D Lasic
Jan 1, 1991·Pharmaceutical Research·J F ScieszkaM J Cho
May 22, 1990·Biochemistry·R M StraubingerK L Hong
Sep 1, 1996·Nuclear Medicine Communications·W J OyenF H Corstens
Dec 31, 1997·Plastic and Reconstructive Surgery·C WangM A Stotland

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Citations

Jan 15, 2010·Plastic and Reconstructive Surgery·Franck M P LeclèreSerge R Mordon
Jul 18, 2006·Microvascular Research·Jae Hong JeongKoji Okamoto
Mar 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Milan T MakaleDavid A Gough
Dec 16, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Iren ConstantinescuMaria Gyongyossy-Issa

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