PMID: 9165350May 1, 1997Paper

In vivo binding and hearing loss after intracochlear infusion of KHRI-3 antibody

Hearing Research
Thankam S NairThomas E Carey

Abstract

The IgG1 mouse monoclonal antibody (MAb) KHRI-3, binds to an antigen of 65-68 kDa expressed on inner ear supporting cells in guinea pigs. We previously showed [Nair et al. (1995) Monoclonal antibody induced hearing loss. Hear. Res. 83, 101-113] that mice carrying the KHRI-3 hybridoma develop high frequency hearing loss and loss of hair cells in the basal turn suggesting that this MAb causes immune-mediated sensorineural hearing loss. To evaluate the specificity of this effect, sterile KHRI-3 and control IgG1 preparations were infused directly into the guinea pig cochlea using Alzet mini-osmotic pumps. Assessments included: (1) hearing, measured by click auditory brain stem responses (ABRs); (2) in vivo antibody binding; and (3) the structural integrity of the organ of Corti. Nine animals were infused with KHRI-3 preparations and 5 controls were infused with control IgG1. Four guinea pigs given KHRI-3 developed 25-55 dB hearing loss. Control animals showed no difference from baseline. In vivo binding of KHRI-3 was detected in the organ of Corti in 6 of the 9 animals, including all 4 that had hearing loss. No staining was observed with control antibody. Confocal microscopy revealed that the in vivo KHRI-3 antibody binding pattern...Continue Reading

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Citations

Oct 11, 2007·Journal of the Association for Research in Otolaryngology : JARO·P K KommareddiT E Carey
Jul 29, 2010·The Protein Journal·P K KommareddiT E Carey
Feb 17, 2000·Hearing Research·D M Prieskorn, J M Miller
Oct 3, 2007·The Journal of Laryngology and Otology·D LoboR Ramírez-Camacho
Feb 1, 2008·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·Saumil N MerchantJoe C Adams
Dec 21, 2000·Current Rheumatology Reports·J T Roland
May 15, 2007·Acta otorrinolaringológica española·José Ramón García BerrocalRafael Ramírez-Camacho
Jul 31, 2007·Hearing Research·Alec N SaltStefan K Plontke
Oct 16, 2015·Journal of the Association for Research in Otolaryngology : JARO·Pavan KommareddiThomas E Carey
Mar 26, 2009·The Laryngoscope·Pavan K KommareddiThomas E Carey
Oct 22, 2014·World Journal of Methodology·David R LoboRafael Ramírez-Camacho
Sep 21, 2004·Current Opinion in Otolaryngology & Head and Neck Surgery·Michael J Ruckenstein
May 14, 2005·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Farnoosh NoushiStephen O'Leary
Jan 18, 2006·Current Opinion in Neurology·Charlotte Agrup, Linda M Luxon
Oct 2, 2003·The Laryngoscope·Kristen YeomT E Carey

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