In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Biochemical and Biophysical Research Communications
Boo-Ja LeeKyung-Hee Paek

Abstract

We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

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Citations

Jan 27, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Khairul I AnsariFiona M Doohan
Nov 7, 2008·Molecular Genetics and Genomics : MGG·Véronique GruberMartin Crespi
Apr 22, 2008·Trends in Plant Science·Katia SchützeChristina Chaban
Oct 31, 2006·Biochemical and Biophysical Research Communications·Boo-Ja LeeKyung-Hee Paek
Sep 26, 2020·Frontiers in Plant Science·Yan YuErxu Pi

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