Abstract
In vivo biocompatibility of nanofibrous poly-L-lactic acid (P), poly-L-lactic acid/gelatin (PG), poly-L-lactic acid/hydroxyapatite (PH), and poly-L-lactic acid/gelatin/hydroxyapatite (PGH) scaffolds, useful in regenerative medicine and drug delivery, was evaluated by subcutaneous implantation in both male and female rats (n = 5) for up to 90 days. The body weight of each animal in the study was evaluated on a weekly basis, and no significant difference was noticed. Total and differential leukocyte counts displayed no inflammatory reaction due to scaffold implantation. Gross observation and histology of necropsied vital organs exhibited normal morphology of cell types and tissue, denying any systemic adverse reaction on distal organs. Histology of subcutaneous tissue surrounding scaffolds was done to assess any local toxic effect of implants and found all scaffolds to be compatible and nontoxic. Moreover, no remnant of scaffolds was observed in any of the histological sections, suggesting all scaffolds to be biodegradable. All the results in this study confirm that nanofibrous scaffolds P, PG, PH, and PGH are biocompatible and safe for bone tissue engineering application.
References
Dec 1, 1992·Journal of Biomedical Materials Research·Y MatsusueY Ikada
Nov 1, 1991·Biomaterials·J KlompmakerA J Pennings
Mar 1, 1983·Journal of Biomedical Materials Research·S R Taylor, D F Gibbons
Apr 1, 1993·Biomaterials·P RobertM Vert
Dec 1, 1995·Journal of Biomedical Materials Research·J H BraukerR C Johnson
Sep 1, 1996·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·M H HofbauerM L Scripps
Jun 11, 1998·Trends in Biotechnology·B S Kim, D J Mooney
Jul 11, 1998·Journal of Biomedical Materials Research·K A HooperJ Kohn
Nov 9, 2000·Biomaterials·Y H AnR J Friedman
Jun 21, 2001·ANZ Journal of Surgery·W R MooreG I Bain
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·Yuji YinKangde Yao
Dec 9, 2004·Biomaterials·F YangS Ramakrishna
Apr 1, 2006·Biomaterials·Hyung Kil NohByung-Moo Min
Jul 21, 2006·Biomaterials·Kyung Mi WooPeter X Ma
Feb 19, 2008·Biomaterials·Travis J Sill, Horst A von Recum
May 27, 2008·Biomaterials·Yongzhong WangDavid L Kaplan
Aug 1, 2008·Biomaterials·Nora E PaulGabriele Zwadlo-Klarwasser
Aug 13, 2009·Biomedical Materials·Sung Eun KimIl Keun Kwon
Jun 25, 2010·Journal of Biomedical Materials Research. Part a·Jinku KimLichun Lu
Oct 13, 2011·BioTechniques·Ryan M BoehlerLonnie D Shea
Nov 3, 2011·PloS One·Ganpat J DaheJayesh R Bellare
Mar 23, 2012·Biomaterials·Hajar SeyednejadWim E Hennink
Apr 3, 2012·Journal of Biomedical Materials Research. Part a·Bit Na LeeMoon Suk Kim
Jul 10, 2012·Biomaterials·Wei JiJohn A Jansen
Citations
Aug 12, 2017·Neurourology and Urodynamics·Mahshid VashaghianJan-Paul Roovers
Dec 6, 2017·Journal of Biomaterials Science. Polymer Edition·A S Sumayya, G Muraleedhara Kurup
Sep 26, 2017·Biomedical Materials·Ajay SuryavanshiRohit Srivastava
Jan 29, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Armin HassanzadehMarziyeh Aghazadeh