In Vivo Bioluminescence Imaging in a Rabbit Model of Orthopaedic Implant-Associated Infection to Monitor Efficacy of an Antibiotic-Releasing Coating

The Journal of Bone and Joint Surgery. American Volume
Robert J MillerLloyd S Miller

Abstract

In vivo bioluminescence imaging (BLI) provides noninvasive monitoring of bacterial burden in animal models of orthopaedic implant-associated infection (OIAI). However, technical limitations have limited its use to mouse and rat models of OIAI. The goal of this study was to develop a larger, rabbit model of OIAI using in vivo BLI to evaluate the efficacy of an antibiotic-releasing implant coating. A nanofiber coating loaded with or without linezolid-rifampin was electrospun onto a surgical-grade locking peg. To model OIAI in rabbits, a medial parapatellar arthrotomy was performed to ream the femoral canal, and a bright bioluminescent methicillin-resistant Staphylococcus aureus (MRSA) strain was inoculated into the canal, followed by retrograde insertion of the coated implant flush with the articular surface. In vivo BLI signals were confirmed by ex vivo colony-forming units (CFUs) from tissue, bone, and implant specimens. In this rabbit model of OIAI (n = 6 rabbits per group), implants coated without antibiotics were associated with significantly increased knee width and in vivo BLI signals compared with implants coated with linezolid-rifampin (p < 0.001 and p < 0.05, respectively). On day 7, the implants without antibiotics wer...Continue Reading

References

Apr 9, 2004·The New England Journal of Medicine·Rabih O Darouiche
Oct 16, 2004·The New England Journal of Medicine·Werner ZimmerliPeter E Ochsner
Apr 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Steven KurtzMichael Halpern
Apr 4, 2007·The Journal of Bone and Joint Surgery. American Volume·Rabih O DarouicheGlenn C Landon
Aug 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dan LiEdward M Schwarz
Aug 21, 2009·The New England Journal of Medicine·Jose L Del Pozo, Robin Patel
Oct 20, 2010·FEMS Microbiology Reviews·Nuria AndreuSiouxsie Wiles
Mar 30, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nicholas M BernthalLloyd S Miller
Aug 13, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jonathan R PribazLloyd S Miller
Aug 26, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Anton H HosmanDaniëlle Neut
Oct 4, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tapan K Dash, V Badireenath Konkimalla
May 5, 2012·The Journal of Arthroplasty·Steven M KurtzJavad Parvizi
Jul 20, 2012·The Journal of Bone and Joint Surgery. American Volume·Brian R WolfPeter Cram
Sep 27, 2012·JAMA : the Journal of the American Medical Association·Peter CramBrian R Wolf
Dec 12, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Douglas R OsmonUNKNOWN Infectious Diseases Society of America
Apr 18, 2014·The Journal of Bone and Joint Surgery. American Volume·Steven M KurtzKevin J Bozic
Jul 6, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John J VarroneEdward M Schwarz
Oct 12, 2015·Orthopaedics & Traumatology, Surgery & Research : OTSR·L GatinA-C Crémieux
Apr 27, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eric M DworskyNicholas M Bernthal
Jul 22, 2016·The Journal of Bone and Joint Surgery. American Volume·Alexandra I StavrakisNicholas M Bernthal
Oct 9, 2016·Advances in Experimental Medicine and Biology·Arianna Barbara LovatiLorenzo Drago
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alyssa G AshbaughLloyd S Miller
Dec 10, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Karen L de Mesy BentleyEdward M Schwarz
Dec 13, 2016·The Journal of the American Academy of Orthopaedic Surgeons·Yu WangLloyd S Miller
Apr 19, 2017·The Journal of Bone and Joint Surgery. American Volume·John M ThompsonLloyd S Miller
Apr 20, 2017·Nature Reviews. Microbiology·András N SpaanVictor J Torres
Oct 19, 2017·The Journal of Bone and Joint Surgery. American Volume·Vishal HegdeNicholas M Bernthal
Jan 23, 2018·Nature Biomedical Engineering·V J SuhardiE Oral

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Citations

Jul 26, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John M Thompson, Lloyd S Miller
May 26, 2020·Advanced Healthcare Materials·María Vallet-RegíIsabel Izquierdo-Barba
Sep 10, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Jorrit W A SchoenmakersMarleen van Oosten
Jun 27, 2020·Disease Models & Mechanisms·Oren GordonSanjay K Jain
Dec 19, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Juliana C QuartermanAliasger K Salem
Sep 18, 2021·Nature Communications·Weixian XiTatiana Segura

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