In vivo brain delivery of v-myc overproduced human neural stem cells via the intranasal pathway: tumor characteristics in the lung of a nude mouse

Molecular Imaging
Eun Seong LeeDong Soo Lee

Abstract

We aimed to monitor the successful brain delivery of stem cells via the intranasal route and to observe the long-term consequence of the immortalized human neural stem cells in the lungs of a nude mouse model. Stably immortalized HB1.F3 human neural stem cells with firefly luciferase gene (F3-effluc) were intranasally delivered to BALB/c nude mice. Bioluminescence images were serially acquired until 41 days in vivo and at 4 hours and 41 days ex vivo after intranasal delivery. Lungs were evaluated by histopathology. After intranasal delivery of F3-effluc cells, the intense in vivo signals were detected in the nasal area, migrated toward the brain areas at 4 hours (4 of 13, 30.8%), and gradually decreased for 2 days. The brain signals were confirmed by ex vivo imaging (2 of 4, 50%). In the mice with initial lung signals (4 of 9, 44.4%), the lung signals disappeared for 5 days but reappeared 2 weeks later. The intense lung signals were confirmed to originate from the tumors in the lungs formed by F3-effluc cells by ex vivo imaging and histopathology. We propose that intranasal delivery of immortalized stem cells should be monitored for their successful delivery to the brain and their tumorigenicity longitudinally.

References

Nov 24, 2004·Investigative Ophthalmology & Visual Science·Stefan ArnholdUlrich Schraermeyer
Mar 28, 2009·European Journal of Cell Biology·Lusine DanielyanWilliam H Frey
Apr 14, 2009·Trends in Pharmacological Sciences·Olle Lindvall, Zaal Kokaia
Jun 22, 2010·Nature Materials·Tomonori NochiHiroshi Kiyono
Jul 15, 2011·Stem Cell Reviews and Reports·Ji-Seon LeeHyuk Jin Cha
Aug 5, 2011·International Journal of Biological Sciences·Weihua WuLi Shen
Sep 6, 2012·International Journal of Biological Sciences·Anja TorsvikRolf Bjerkvig
Jan 4, 2013·Stem Cells Translational Medicine·Matthias ReitzNils Ole Schmidt

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Citations

Sep 27, 2018·Journal of the National Cancer Institute·Litia A CarvalhoBakhos A Tannous
Jan 10, 2018·Evolution; International Journal of Organic Evolution·Vivek JayaswalJosé M Ranz

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Methods Mentioned

BETA
bioluminescence
transfection

Software Mentioned

SPSS

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