In vivo brain glucose measurements: differential normal pulse voltammetry with enzyme-modified carbon fiber microelectrodes

Analytical Chemistry
L I NetchiporoukRaymond Cespuglio

Abstract

The enzyme glucose oxidase was immobilized on the surface of carbon fiber microelectrodes (CFMEs) either by cross-linking in glutaraldehyde vapor or by enzyme entrapment in electropolymerized films of m-phenylenediamine or resorcinol. The cross-linked enzymatic layer was, in the given conditions, covered with an additional membrane of Nafion or cellulose acetate. The prepared glucose sensors were tested using differential normal pulse voltammetry (DNPV, in which the scan comprises successive double pulses ("prepulse and pulse"), the prepulses are of increasing amplitude, and the current measured is the differential of the current existing between each prepulse and pulse). With properly chosen DNPV parameters, the response to glucose presented a peak at a potential of about 1 V versus an Ag/AgC1-reference, owing to the oxidation of enzymatically produced hydrogen peroxide. The calibration curves obtained (peak height/glucose concentration) were linear from 0.3-0.5 up to 1.5-6.5 mM and showed a sensitivity ranging from 1.4 up to 34.5 mA M-1 cm-2, depending on the sensor type. The DNPV response to glucose exhibited an essential insensitivity toward easily oxidizable interfering substances such as ascorbic acid and acetaminophen pr...Continue Reading

References

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Citations

Jan 29, 2013·Applied Biochemistry and Biotechnology·Anish KhanSulaiman Ab Ghani
Dec 24, 2013·Biosensors & Bioelectronics·Wenjing WeiXinxia Cai
Jul 2, 2003·Biosensors & Bioelectronics·Won Jun Sung, You Han Bae
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Aug 24, 2002·The European Journal of Neuroscience·Nataliya ShramRaymond Cespuglio
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Feb 16, 2013·Enzyme and Microbial Technology·Juanjuan ZhangXia Zhong
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Oct 4, 2012·Physical Chemistry Chemical Physics : PCCP·Domhnall MacAodhaDónal Leech
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Nov 18, 2003·Analytical Chemistry·Rong-Sheng ChenJie-Ke Cheng
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