In vivo brain phosphocreatine and ATP regulation in mice fed a creatine analog

The American Journal of Physiology
D HoltzmanF Jensen

Abstract

Mitochondrial and cytosolic creatine kinase (CK) isozymes are active in cells with high and variable ATP metabolic rates. beta-Guanidinopropionic acid (GPA), a competitive inhibitor of creatine transport, was used to study the hypothesis that the creatine-CK-phosphocreatine (PCr) system is important in regulating brain ATP metabolism. The CK-catalyzed reaction rate and reactant concentrations were measured in vivo with 31P nuclear magnetic resonance spectroscopy during energy deficit (hypoxia) or high-energy turnover (seizures) states in urethane-anesthetized mice fed GPA, creatine, or standard chow (controls). Brain phosphagen (i.e., cellular energy reserves) or PCr plus phosphorylated GPA (GPAP) concentrations were equal. The phosphagen-to-NTP ratio was lower than in controls. In vivo CK reaction rate decreased fourfold, whereas ex vivo CK activity that was biochemically measured was doubled. During seizures, CK-catalyzed fluxes increased only in GPA-fed mice. Phosphagen increased in GPA-fed mice, whereas PCr decreased in controls. Survival was higher and brain phosphagen and ATP losses were less for hypoxic GPA-fed mice than for controls. In contrast to mice fed GPA, hypoxic survival and CK reactant concentrations during hyp...Continue Reading

References

Sep 25, 1992·Biochimica Et Biophysica Acta·M WyssT Wallimann
Jan 1, 1992·Annual Review of Biochemistry·D C Wallace
Feb 22, 1991·Brain Research. Developmental Brain Research·D HoltzmanL J Neuringer
Feb 29, 1988·Biochemical and Biophysical Research Communications·J P HossleJ C Perriard
Dec 1, 1974·The American Journal of Physiology·D C HowseF Plum
Feb 1, 1984·Quarterly Reviews of Biophysics·J R Alger, R G Shulman
Dec 1, 1981·Journal of Neurochemistry·T S Whittingham, P Lipton
Aug 1, 1984·Annals of Neurology·O A PetroffR G Shulman
Apr 18, 1995·Brain Research. Developmental Brain Research·M TsujiD Holtzman
Jan 1, 1993·Developmental Neuroscience·D HoltzmanW Hemmer
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M StadhoudersT Wallimann
Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D HoltzmanD Jacobs
Jan 1, 1996·Developmental Neuroscience·M D SaltarelliR D Blakely
Jan 1, 1996·Developmental Neuroscience·D HoltzmanR Meyers
Aug 1, 1961·Journal of Neurochemistry·R N LOLLEYF E SAMSON

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Citations

Feb 24, 2001·Journal of Neurochemistry·N BrustovetskyJ M Dubinsky
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk
Jun 30, 2006·American Journal of Physiology. Cell Physiology·Mordhwaj S Parihar, Gregory J Brewer
Aug 9, 2003·Pediatric Research·Anne-Marie Van Cappellen Van WalsumHenk W Jongsma
Dec 22, 1999·Journal of Inherited Metabolic Disease·M ValtonenO Simell
Apr 7, 2000·Journal of the American Society of Nephrology : JASN·Aki HirayamaJohn S Hothersall
Dec 18, 2001·Journal of Neuroscience Research·T KekelidzeD Holtzman

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