In Vivo Capture of Circulating Tumor Cells Based on Transfusion with a Vein Indwelling Needle

ACS Applied Materials & Interfaces
Hongyan ZhangBo Tang

Abstract

Detection of circulating tumor cells (CTCs) could be used as a "liquid biopsy" for tracking the spread of cancer. In vitro detection methods based on blood sampling and in vitro CTC capture often suffer from the small sampling volume and sampling error. Here, the in vivo capture of CTCs based on transfusion with a surface-modified vein indwelling needle is proposed. When the needle was applied to transfusion in the vein, the simultaneous capture of CTCs was performed. To investigate the actual capture efficiency of the in vivo capture method, labeled MCF-7 cells were directly injected into the veins of rabbits, wild type mice, and nude mice and could be successfully captured. Two of 5 MCF-7 cells injected into the veins of nude mice were successfully captured. To investigate the CTC capture of mouse tumor model and compare with the in vitro method, mice were subcutaneous inoculated with metastatic 4T1 cells. Seven and 21 days after inoculation, CTCs were captured for the first time using in vivo and in vitro methods, respectively. This predicted that the in vivo method could be more suitable for use of early diagnosis of cancer than the in vitro method. As CTC capture can be performed at the same time as transfusion and does no...Continue Reading

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Citations

May 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chih-Sheng ChiangSan-Yuan Chen
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
Apr 11, 2017·Chemical Society Reviews·Zheyu ShenXiaoyuan Chen
Jul 19, 2017·Scientific Reports·Gwangseong KimAngelo Gaitas
May 29, 2021·Light, Science & Applications·Xi ZhuXunbin Wei
Feb 18, 2017·Analytical Chemistry·Frank D ScheragJürgen Rühe
Mar 13, 2020·Analytical Chemistry·Shi-Bo ChengWei-Hua Huang

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